Protective Effects of Kaempferol on D-Ribose-Induced Mesangial Cell Injury
Protective Effects of Kaempferol on D-Ribose-Induced Mesangial Cell Injury
复制标题
山奈酚对 D-核糖诱导的系膜细胞损伤的保护作用
DOI：
10.1155/2019/7564207
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发表时间：
2019-03
影响因子：
--
通讯作者：
Wang Xuemei
中科院分区：
文献类型：
--
作者：
Zhang Ning;Zhao Shaoyang;Hong Jinni;Li Weiwei;Wang Xuemei
近年来研究发现2型糖尿病患者尿D-核糖水平明显高于同龄正常人，且D-核糖比D-葡萄糖更易发生糖基化反应，并诱导氧化应激。山奈酚是黄芪中的主要活性成分之一，具有抗氧化等多种生理活性。本研究探讨了山奈酚对D-核糖处理的系膜细胞的保护作用。CCK-8法和LDH法检测细胞活力和细胞毒性。免疫荧光和流式细胞术检测AGE的形成和ROS的积累。测定GSH水平以反映抗氧化性。Hoechst 33258染色、AO/EB染色和western blot检测细胞凋亡。JC-1染色、Western blot和RT-PCR检测线粒体膜完整性。western blot检测细胞自噬水平的变化。结果表明，D-核糖不仅诱导细胞损伤，增加AGE的形成和ROS的积累，而且还导致GSH耗竭。进一步的研究表明，D-核糖诱导线粒体去极化和caspase-9/3的激活。但山奈酚可以部分阻止这些损害。随后，证实了山奈酚修复了D-核糖诱导的自噬紊乱，3-MA可逆转D-核糖条件下山奈酚的保护作用。我们的研究表明，D-核糖可诱导系膜细胞内AGE的积累和ROS的产生，并引起线粒体凋亡，而山奈酚可减轻这些变化，其保护作用可能与自噬的修复有关。
Recently, it has been found that the level of urinary D-ribose in type 2 diabetes is notably higher than that in age-matched normal control, and D-ribose is more reactive in the glycation than D-glucose and induces oxidative stress. Kaempferol is one of the main bioactive components in Astragalus membranaceus, with numerous physiological actives, such as antioxidant. The present study investigated the protective effects of kaempferol on D-ribose-treated mesangial cells. CCK-8 and LDH assay were used to test cell viability and cell toxicity. Immunofluorescence and flow cytometry were used to detect the AGE formation and ROS accumulation. GSH level was measured to reflect oxidation resistance. Cell apoptosis was evaluated by Hoechst 33258 staining, AO/EB staining, and western blot. Mitochondrial membrane integrity was detected by JC-1 staining, western blot, and RT-PCR. The change of autophagy level was tested by western blot. The results indicated that D-ribose induced not only cell damage and increased AGE formation and ROS accumulation but also GSH depletion. Further studies demonstrated that D-ribose induced mitochondrial depolarization and the activation of caspase-9/3. But kaempferol could partly block these damages. Subsequently, it was confirmed that kaempferol repaired the autophagy disturbance induced by D-ribose, and 3-MA could reverse the protective effect of kaempferol under D-ribose condition. Our study demonstrated that D-ribose induced AGE accumulation and ROS production in mesangial cell and caused mitochondrial apoptosis, but kaempferol could attenuate these changes and its protective effect might be related to the repair of autophagy.
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影响因子：
7.2
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5
作者：
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通讯作者：
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