Effect of pancreatic polypeptide on rat dorsal vagal complex neurons.
Effect of pancreatic polypeptide on rat dorsal vagal complex neurons.
复制标题
胰多肽对大鼠背侧迷走神经复合体神经元的影响。
DOI：
10.1113/jphysiol.1997.sp021942
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Rogers,RC
中科院分区：
文献类型：
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作者：
McTigue,DM;Hermann,GE;Rogers,RC
1. 胰腺多肽（PP）微注入迷走神经背复合体（DVC）通过迷走神经机制提高胃活动。因此，我们假设PP改变了构成DVC的核的活性，即孤束核（NTS）和背侧运动核（DMN）。2. 采用体内和体外方法。在体内研究中，使用微移液管记录和注射载体或PP。在迷走神经刺激后，分别使用正位和反位激活方法将神经元识别为NTS或DMN。胃相关DVC神经元的定位采用胃窦充气法。在体外研究中，从髓质切片记录DMN神经元。3. 在鉴定的28个NTS和DMN神经元中，PP微注射后15个被激活，6个被抑制，7个未受影响。42个胃相关神经元位于DVC，其中25个受到PP刺激，17个没有变化。未发现胃相关细胞受到抑制。4. 在体外实验中，66%的DMN神经元被PP激活（n = 27/47），其余33%的DMN神经元被抑制（n = 14/47）。在正常或突触阻断介质中也得到类似的结果。5. 这些结果支持了PP改变DVC神经元活动的假设，这可能导致先前观察到的胃活动的改变。
1. Pancreatic polypeptide (PP) microinjected into the dorsal vagal complex (DVC) elevates gastric activity through a vagal mechanism. Thus, it was hypothesized that PP alters the activity of nuclei comprising the DVC, i.e. the nucleus tractus solitarii (NTS) and the dorsal motor nucleus (DMN). 2. In vivo and in vitro approaches were used. For in vivo studies, micropipettes were used for recording and injecting vehicle or PP. Neurons were identified as NTS or DMN using orthodromic and antidromic activation, respectively, following vagal stimulation. Gastric‐related DVC neurons were located using antral inflation. For in vitro studies, DMN neurons were recorded from medullary slices. 3. Of the twenty‐eight NTS and DMN neurons identified, fifteen were activated, six inhibited and seven unaffected after PP microinjection. Forty‐two gastric‐related neurons were located in the DVC, of which twenty‐five were stimulated by PP and seventeen exhibited no change. No gastric‐related cells were inhibited. 4. For in vitro studies, 66% of DMN neurons were activated by PP (n = 27/47) while the remaining 33% were inhibited (n = 14/47). Similar results were obtained in normal or synaptic blockade media. 5. These results support the hypothesis that PP alters DVC neuronal activity, which may thereby lead to the previously observed alterations in gastric activity.