Molecular characterization and expression analysis of complement component C3 in southern catfish (Silurus meridionalis) and a whole mount in situ hybridization study on its ontogeny
Molecular characterization and expression analysis of complement component C3 in southern catfish (Silurus meridionalis) and a whole mount in situ hybridization study on its ontogeny
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南方鲶补体成分C3的分子表征、表达分析及其个体发育的整体原位杂交研究
DOI：
10.1016/j.fsi.2018.10.083
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发表时间：
2019-01-01
影响因子：
4.7
通讯作者：
Zhang, Qi-Zhong
中科院分区：
文献类型：
--
作者：
Fu, Yao-Wu;Zhu, Cheng-Ke;Zhang, Qi-Zhong
补体系统在保护鱼类生命早期免受病原体攻击方面发挥着重要作用。补体成分 C3 是补体系统的核心成分。本工作旨在克隆南方鲶（Silurus meridionalis）C3 cDNA全长序列，检测C3的组织表达模式，研究C3在胚胎和幼虫中的个体发育，并评估C3在病原体感染反应中的表达。从南方鲶中克隆了全长5157 bp的C3 cDNA序列和4938 bp的开放阅读框（ORF）。推导的氨基酸序列与其他硬骨鱼相似。采用RT-PCR和原位杂交技术检测肝、脾、胃、肠、头肾中C3 mRNA的表达。整体原位杂交结果显示，C3 首先在受精后 34 小时 (hpf) 的卵黄合胞体层中表达，随后在孵化后 36 小时 (hph) 的肝脏中表达。当用嗜水气单胞菌攻击时，24小时时肝脏和脾脏中C3的转录物表现出显着上调。结果表明，补体C3在南方鲶鱼早期发育阶段防御入侵病原体方面发挥着关键作用。因此，这些结果为了解C3在鲶形目鱼类早期发育过程中的功能提供了重要信息。
The complement system plays an important role in protecting fish against attack by pathogens early in life. Complement component C3 is a central component in the complement system. The present work aimed to clone the full length C3 cDNA sequence of southern catfish (Silurus meridionalis), detect the tissue expression patterns of C3, investigate the ontogeny of C3 in embryo and larva, and assess the expression of C3 in response to pathogen infection. The full length C3 cDNA sequence of 5157 bp with an open reading frame (ORF) of 4938 bp was cloned from southern catfish. The deduced amino acid sequence showed similarity with other teleost fish. The mRNA expression of C3 was detected in liver, spleen, stomach, intestine, and head kidney with RT-PCR and in situ hybridization. Whole mount in situ hybridization results revealed that C3 was first expressed in the yolk syncytial layer at 34 h post fertilization (hpf), followed by the liver at 36 h post hatching (hph). When challenged with Aeromonas hydrophila, the transcripts of C3 showed a significant up-regulation in liver and spleen at 24 h. The results suggested that complement C3 played a key role in defense against invading pathogens in the early development stages of southern catfish. Therefore, these results provide important information to understand the functions of C3 during fish early development in Siluriformes.