CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection
CD4+ T, but not CD8+ or B, lymphocytes mediate facial motoneuron survival after facial nerve transection
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DOI：
10.1016/s0889-1591(03)00028-x
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发表时间：
2003-10-01
影响因子：
15.1
通讯作者：
Jones, KJ
中科院分区：
文献类型：
--
作者：
Serpe, CJ;Coers, S;Jones, KJ
在缺乏T和B淋巴细胞的免疫缺陷小鼠中，面部运动神经元（FMN）在损伤中存活并成功再生的能力基本上受到损害（Serpe等人，1999年）。本研究的目的是确定哪种T细胞亚群（CD 4+和/或CD 8+），以及B淋巴细胞是否参与神经损伤后FMN的存活。所有小鼠均接受右侧面神经轴突切断术，左侧（未切断）作为内部对照。术后4周测量右侧（切割）FMN存活率，并表示为左侧（未切割）对照侧的百分比。野生型小鼠的FMN存活率为86% +/-1.5。相比之下，CD 4 KO小鼠中的FMN存活率为60% +/-2.0。单独用CD 4 + T细胞重建CD 4 KO小鼠或重组酶激活基因-2敲除（RAG-2 KO）小鼠（其缺乏功能性T和B细胞）使FMN存活恢复至野生型水平（分别为85% +/-1.2和84% +/-2.5）。野生型、CD 8 KO和MmuMT（B细胞缺陷）小鼠之间的FMN存活率没有差异。用单独的CD 8 + T细胞或单独的B细胞重建RAG-2 KO小鼠不能恢复FMN存活水平（分别为65% +/-1.5和63% +/-1.0）。结论：外周神经损伤后FMN的存活是由CD 4 + T淋巴细胞介导的，而不是由CD 8 + T淋巴细胞或B细胞介导的。(C)2003 Elsevier Science（美国）。All rights reserved.
The capacity of facial motor neurons (FMN) to survive injury and successfully regenerate is substantially compromised in immunodeficient mice, which lack T and B lymphocytes (Serpe et al., 1999). The goal of the present study was to determine which T cell subset (CD4+ and/or CD8+), and whether the B lymphocyte, is involved in FMN survival after nerve injury. All mice were subjected to a right facial nerve axotomy, with the left (uncut) side serving as an internal control. FMN survival, of the right (cut) side, was measured 4 weeks post-operative, and expressed as a percentage of the left (uncut) control side. FMN survival in wild-type mice was 86% +/- 1.5. In contrast, FMN survival in CD4 KO mice was 60% +/- 2.0. Reconstitution of either CD4 KO mice, or recombinase activating gene-2 knockout (RAG-2 KO) mice (which lack functional T and B cells) with CD4+ T cells alone restored FMN survival to wild-type levels (85% +/- 1.2 and 84% +/- 2.5, respectively). There was no difference in FMN survival between wild-type, CD8 KO and MmuMT (B cell deficient) mice. Reconstitution of RAG-2 KO mice with CD8+ T cells alone, or B cells alone, failed to restore FMN survival levels (65% +/- 1.5 and 63% +/- 1.0, respectively). It is concluded that, of the population of FMN that do not survive injury, CD4+ T lymphocytes, but not CD8+ T lymphocytes or B cells, mediate FMN survival after peripheral nerve injury. (C) 2003 Elsevier Science (USA). All rights reserved.