Basic characteristics of plasma rich in growth factors (PRGF): blood cell components and biological effects.
Basic characteristics of plasma rich in growth factors (PRGF): blood cell components and biological effects.
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DOI：
10.1002/cre2.26
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发表时间：
2016-11
影响因子：
1.8
通讯作者：
Kawase T
中科院分区：
文献类型：
--
作者：
Nishiyama K;Okudera T;Watanabe T;Isobe K;Suzuki M;Masuki H;Okudera H;Uematsu K;Nakata K;Kawase T
富血小板血浆（PRP）由于其高浓度的各种生长因子和血小板而广泛用于再生医学。然而，在PRP或其他PRP衍生物中尚未研究血细胞组分的分布。在这项研究中，我们集中在血浆中富含生长因子（PRGF），PRP衍生物，并分析了血小板和白色血细胞（WBC）的分布。从健康志愿者（N = 14）收集外周血样品并离心以制备PRGF和PRP。使用自动血液分析仪计数血细胞。使用人骨膜细胞测定PRP和PRGF制剂对细胞增殖的影响。在PRGF制剂中，红细胞和WBC几乎完全消除，血小板浓缩了2.84倍，而在PRP制剂中，血小板和WBC分别浓缩了8.79倍和5.51倍。PRGF制剂中的血小板计数与全血样品中的血小板计数呈正相关，而血小板浓缩率与全血样品中的红细胞计数呈负相关。相比之下，PRP制剂中的血小板计数和浓缩率受到全血样本中WBC计数的显著影响。PRP制剂，但不是PRGF制剂，在体外以较高剂量显著抑制细胞生长。因此，这些结果表明，PRGF制剂可以通过包含WBC和体外骨膜细胞增殖的剂量依赖性刺激与PRP制剂明确区分。
Platelet‐rich plasma (PRP) is widely used in regenerative medicine because of its high concentrations of various growth factors and platelets. However, the distribution of blood cell components has not been investigated in either PRP or other PRP derivatives. In this study, we focused on plasma rich in growth factors (PRGF), a PRP derivative, and analyzed the distributions of platelets and white blood cells (WBCs). Peripheral blood samples were collected from healthy volunteers (N = 14) and centrifuged to prepare PRGF and PRP. Blood cells were counted using an automated hematology analyzer. The effects of PRP and PRGF preparations on cell proliferation were determined using human periosteal cells. In the PRGF preparations, both red blood cells and WBCs were almost completely eliminated, and platelets were concentrated by 2.84‐fold, whereas in the PRP preparations, both platelets and WBCs were similarly concentrated by 8.79‐ and 5.51‐fold, respectively. Platelet counts in the PRGF preparations were positively correlated with platelet counts in the whole blood samples, while the platelet concentration rate was negatively correlated with red blood cell counts in the whole blood samples. In contrast, platelet counts and concentration rates in the PRP preparations were significantly influenced by WBC counts in whole blood samples. The PRP preparations, but not the PRGF preparations, significantly suppressed cell growth at higher doses in vitro. Therefore, these results suggest that PRGF preparations can clearly be distinguished from PRP preparations by both inclusion of WBCs and dose‐dependent stimulation of periosteal cell proliferation in vitro.