LIPID COMPOSITION OF ERYTHROCYTES IN VARIOUS MAMMALIAN SPECIES
LIPID COMPOSITION OF ERYTHROCYTES IN VARIOUS MAMMALIAN SPECIES
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DOI：
10.1016/0005-2760(67)90152-x
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发表时间：
1967-01-01
期刊：
影响因子：
--
通讯作者：
NELSON, GJ
中科院分区：
文献类型：
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作者：
NELSON, GJ
本文研究了大鼠、兔、猪、狗、马、绵羊、牛、山羊、猫和豚鼠等几种常见哺乳动物红细胞中脂质的分布。从新鲜的、彻底洗涤的完整浓缩细胞中提取脂质。用Sephadex柱层析和薄层层析分离脂质。红外分光光度法也用于鉴定组分。报告了胆固醇、糖脂和磷脂的分布。胆固醇接近。所有物种中总脂质的26%，而磷脂的范围在50%至70%之间。糖脂的变化相当大，占兔红细胞中脂质的5.3%和马红细胞中脂质的23.5%。未详细分析糖脂部分。磷脂被分离成各种类别，但乙烯基或甘油醚化合物均未与二酰基衍生物分离。所有种属共有的磷脂为磷脂酸、磷脂酰乙醇胺、磷脂酰丝氨酸、磷脂酰肌醇和鞘磷脂。然而，这些红细胞磷脂的相对丰度存在明显的物种差异。在绵羊、奶牛或山羊中未检测到卵磷脂。然而，在这些种属中检测到一种尚未鉴别的磷脂。
Lipid distribution in the erythrocyte was investigated in several common mammals, including rat, rabbit, pig, dog, horse, sheep, cow, goat, cat and guinea pig. Lipids were extracted from fresh, thoroughly washed, whole packed cells. The lipids were purified by Sephadex column chromatography and separated by thin-layer chromatography. Infrared spectrophotometry was also used to identify components. The distribution of cholesterol, glycplipids, and the phospholipids is reported. Cholesterol was approx. 26% of total lipid in all species while phospholipids ranged between 50 and 70%. The glycolipids were considerably more variable, accounting for 5.3% of the lipids in the rabbit erythrocyte and 23.5% of the lipids in the horse erythrocyte. The glycolipid fraction was not analyzed in detail. The phospholipids were separated into the various classes, but neither vinyl nor glyceryl ether compounds were separated from diacyl derivatives. The phospholipids common to all species were phosphatidic acid, phosphatidyl ethanolamine, phosphatidyl serine, phosphatidyl inositol, and sphingomyelin. There are, however, marked species variations in the relative abundance of these erythrocyte phospholipids. No lecithin was detected in sheep, cow, or goat. However, an as yet unidentified phospholipid was detected in these species.