Transitional B cells in humans: Characterization and insight from B lymphocyte reconstitution after hematopoietic stem cells transplantation
Transitional B cells in humans: Characterization and insight from B lymphocyte reconstitution after hematopoietic stem cells transplantation
复制标题
DOI：
10.1016/j.clim.2007.11.013
复制
发表时间：
2008-04-01
影响因子：
8.6
通讯作者：
Jacquot, Serge
中科院分区：
文献类型：
--
作者：
Marie-Cardine, Aude;Divay, Florence;Jacquot, Serge
最近已在人外周血中鉴定出过渡性B细胞。然而，它们在人B细胞分化中的确切作用尚未确定。因此，除了在健康成人和儿童的血液以及脐带血中进一步表征它们之外，我们还使用造血干细胞移植（HSCT）后的免疫重建模型。以确定它们在人类B细胞发育中的作用。人移行B细胞被可靠地鉴定为CD 19 + CD 24（高）CD 38（高）淋巴细胞，并代表健康成人外周血中约4%的B细胞。相反，它们在脐带血中含量丰富（接近B细胞的50%），并且在婴儿期其百分比逐渐降低。同样，HSCT后。外周血中最先出现的B细胞为过渡型B细胞，其后过渡型B细胞比例逐渐下降白色成熟幼稚型B细胞比例逐渐上升。我们的研究结果正式证明了过渡型B细胞是人类成熟B细胞发育所必需的中间体。一代(C)2007年Etsevier Inc. All rights reserved.
Transitional B cells have been recently identified in human peripheral blood. However, their precise rote in human B cell differentiation has not been established. Therefore, besides characterizing them further in the blood of healthy adults and children and cord blood, we used the immune reconstitution after hematopoietic stem cell transplantation (HSCT) model. to define their role in human B cell development. Human transitional B cells are reliably identified as CD19+ CD24(high) CD38(high) lwymphocytes and represent approximately 4% of B cells in healthy adult peripheral blood. In contrast, they are abundant in cord blood (near 50% of B cells) and their percentage progressively decreases during infancy. Similary, after HSCT all. B cells first appearing in peripheral blood are transitional B cells; afterwards, the transitional B cell percentage progressively decreases white the mature naive B cell proportion rises. Our results now formally demonstrate that transitional B cells are necessary developmental intermediates for human mature B cell. generation. (C) 2007 Etsevier Inc. All rights reserved.