EFFECT OF LEUKEMIA AND METHOTREXATE ON DIGESTIVE ENZYMES IN THE JEJUNUM OF MICE
EFFECT OF LEUKEMIA AND METHOTREXATE ON DIGESTIVE ENZYMES IN THE JEJUNUM OF MICE
复制标题
DOI：
10.1159/000198589
复制
发表时间：
1981-01-01
期刊：
影响因子：
3.2
通讯作者：
HATCH, TF
中科院分区：
文献类型：
--
作者：
BRANSKI, D;LEBENTHAL, E;HATCH, TF
采用白血病小鼠模型来阐明白血病和细胞毒性药物对空肠粘膜及其相关消化酶的单独影响。与正常小鼠相比，白血病小鼠的有丝分裂活性、隐窝深度和绒毛-隐窝商没有显着变化。白血病小鼠服用20 mg[抗肿瘤药物]甲氨蝶呤（MTX）/kg后48小时，有丝分裂活性和隐窝深度显着降低（P < 0.01）。与非白血病对照相比，白血病小鼠空肠中的蔗糖酶（P < 0.001）和麦芽糖酶（P < 0.025）活性显着升高。在非白血病和白血病小鼠中，MTX 给药的剂量反应曲线显示，在 20 mg/kg 剂量下，蔗糖酶 (P < 0.001) 和麦芽糖酶 (P < 0.0025) 活性显着降低并降至最低点。因此，在小鼠模型中，白血病本身不会导致小肠功能显着下降。在小肠中，MTX 似乎导致隐窝细胞有丝分裂活性、隐窝深度降低以及蔗糖酶和麦芽糖酶活性降低。
A leukemic mouse model was employed to elucidate the separate effect of leukemia and cytotoxic drugs on the jejunal mucosa and its associated digestive enzymes. The mitotic activity, depth of the crypt and villus-crypt quotient were not significantly changed in leukemic mice in comparison to normal mice. The mitotic activity and the depth of the crypt 48 h after 20 mg [of the antineoplastic drug] methotrexate (MTX)/kg were significantly reduced (P < 0.01) in leukemic mice. Sucrase (P < 0.001) and maltase (P < 0.025) activities in the jejunum from leukemic mice were significantly elevated in comparison with non-leukemic controls. In non-leukemic and leukemic mice, the dose-response curves for MTX administration revealed a significant decrease and a nadir in sucrase (P < 0.001) and maltase (P < 0.0025) activities at the dosage of 20 mg/kg. Thus, in the mouse model, leukemia per se does not contribute to significant diminution in small intestinal function. In the small intestine MTX appears to be responsible for a decrease in the mitotic activity of crypt cells, depth of the crypt and diminished sucrase and maltase activities.