Enhancing the efficacy of cytotoxic agents for cancer therapy using photochemical internalisation.
Enhancing the efficacy of cytotoxic agents for cancer therapy using photochemical internalisation.
复制标题
使用光化学内在化增强了细胞毒性剂对癌症治疗的疗效。
DOI：
10.1002/ijc.29510
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发表时间：
2016-03-01
影响因子：
6.4
通讯作者：
Woodhams JH
中科院分区：
文献类型：
--
作者：
Martinez de Pinillos Bayona A;Moore CM;Loizidou M;MacRobert AJ;Woodhams JH
光化学内化（PCI）是用于改善某些生物活性剂的细胞递送的技术，所述生物活性剂倾向于在内溶酶体内螯合。有广泛的试剂适用于基于PCI的递送，包括毒素、寡核苷酸、基因和免疫缀合物，这证明了该技术的多功能性。PCI的基本机制涉及使用光敏剂触发药剂从靶细胞内的内溶酶体释放，所述光敏剂选择性地保留在内溶酶体膜上。可见光对光敏剂的激发通过光氧化损伤导致膜的破坏，从而将试剂释放到胞质溶胶中。这种治疗使药物能够更有效地到达其预期的亚细胞靶点，并提高其疗效。在这篇综述中，我们总结了这项技术的应用，重点放在癌症化疗。
Photochemical internalisation (PCI) is a technique for improving cellular delivery of certain bioactive agents which are prone to sequestration within endolysosomes. There is a wide range of agents suitable for PCI‐based delivery including toxins, oligonucleotides, genes and immunoconjugates which demonstrates the versatility of this technique. The basic mechanism of PCI involves triggering release of the agent from endolysosomes within the target cells using a photosensitiser which is selectively retained with the endolysosomal membranes. Excitation of the photosensitiser by visible light leads to disruption of the membranes via photooxidative damage thereby releasing the agent into the cytosol. This treatment enables the drugs to reach their intended subcellular target more efficiently and improves their efficacy. In this review we summarise the applications of this technique with the main emphasis placed on cancer chemotherapy.