MULTIPLE-DRUG RESISTANCE IN MYCOBACTERIUM-AVIUM - IS THE WALL ARCHITECTURE RESPONSIBLE FOR THE EXCLUSION OF ANTIMICROBIAL AGENTS
MULTIPLE-DRUG RESISTANCE IN MYCOBACTERIUM-AVIUM - IS THE WALL ARCHITECTURE RESPONSIBLE FOR THE EXCLUSION OF ANTIMICROBIAL AGENTS
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DOI：
10.1128/aac.20.5.666
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发表时间：
1981-01-01
影响因子：
4.9
通讯作者：
DAVID, HL
中科院分区：
文献类型：
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作者：
RASTOGI, N;FREHEL, C;DAVID, HL
M.特征在于它们在实验室中用于分枝杆菌鉴定的9种生化试验中的阴性反应的禽分枝杆菌，在硝酸还原酶、吐温-80水解、β-葡聚糖酶和β-葡聚糖酶中变为阳性。葡糖苷酶、酸性磷酸酶、碱性磷酸酶、青霉素酶和海藻糖酶，在它们的壁部分被去除以产生原生质球之后。在大多数生化过程中的阴性结果显然是由壁水平的排斥机制引起的。初步的透射和扫描电子显微镜研究表明，在实验室保持不透明，圆顶形（SmD）和主机回收的光滑，透明（SmT）的M菌落类型的变体之间的差异，在壁水平。avium，并建议存在一个外部规则结构层的SmT变体。利用不同的多糖显色方法的比较超微结构研究证实了外多糖层的存在下，SmT的变种，这可能是有关的增强实验动物的致病性和耐药性相比，SmD的变种。本文就其多重耐药性、毒力和基因表达等方面进行了讨论。avium
Whole cells of M. avium, characterized by their negative response in the 9 biochemical tests used for mycobacterial identification in the laboratory, turned positive for nitrate reductase, Tween-80 hydrolysis, .beta.-glucosidase, acid phosphatase, alkaline phosphatase, penicillinase and trehalase after their wall portion was removed to yield spheroplasts. The negative results in most of the biochemical procedures apparently were caused by the exclusion mechanism at the wall level. Preliminary transmission and scanning electron microscopic studies showed differences at wall level between laboratory-maintained opaque, dome-shaped (SmD) and host-recycled smooth, transparent (SmT) colony type variants of M. avium and suggested the presence of an outer regularly structured layer in SmT variants. Comparative ultrastructural studies utilizing different polysaccharide coloration methods confirmed the presence of an outer polysaccharide layer in SmT variants which was probably related to their enhanced pathogenicity for experimental animals and drug resistance as compared to that of SmD variants. These findings are discussed with respect to multiple drug resistance, virulence and gene expression of M. avium.