Cloning, structure, and expression of the mouse Ovca1 gene
Cloning, structure, and expression of the mouse Ovca1 gene
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DOI：
10.1006/bbrc.2001.5488
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发表时间：
2001-09-07
影响因子：
3.1
通讯作者：
Behringer, RR
中科院分区：
文献类型：
--
作者：
Chen, CM;Behringer, RR
我们报告了小鼠Ovca 1基因的分离，该基因是人OVCA 1/DPH 2L 1的直向同源物，是一种与卵巢癌相关的假定肿瘤抑制基因。小鼠Ovca 1基因至少含有13个外显子，长度约为17 kb。北方分析表明Ovca 1在大多数成年小鼠组织中表达。Ovca 1转录本的长度为2.1 kb。RT-PCR分析表明，Ovca 1在胚胎中的表达，从8.5天后coc.（d.p.c.）至10.5 d.p.c.，和14.5d.p.c.的各种器官。胚胎小鼠Ovca 1编码438个氨基酸的蛋白质，与人OVCA 1具有高度同源性。Western blot和免疫组化显示，小鼠OVCA 1是一个50 kDa的蛋白，主要定位在核中的点状图案。基于基因同源性、结构和表达模式，这些发现表明小鼠Ovca 1是人OVCA 1/DPH 2L 1的直向同源物。这项研究将有助于阐明Ovca 1在癌症中的体内作用的实验。(C)北京：科学出版社.
We report the isolation of the mouse Ovca1 gene, the orthologue of human OVCA1/DPH2L1, a putative tumor suppressor associated with ovarian cancer. Mouse Ovca1 contains at least 13 exons and spans approximately 17 kb. Northern analysis showed that Ovca1 is expressed in most adult mouse tissues. The most predominant Ovca1 transcript is 2.1 kb. RT-PCR analysis demonstrated Ovca1 expression in embryos from 8.5 days postcoitum (d.p.c.) to 10.5 d.p.c., and various organs of 14.5 d.p.c. embryos. Mouse Ovca1 encodes a protein of 438 amino acids and has high identity with human OVCA1. Western blot and immunohistochemistry revealed that mouse OVCA1 is a 50-kDa protein that is predominately localized in a punctate pattern in the nucleus. Based on gene homology, structure, and expression patterns, these findings indicate that mouse Ovca1 is the orthologue of human OVCA1/DPH2L1. This study will facilitate experiments to elucidate the in vivo role of Ovca1 in cancer. (C) 2001 Academic Press.