XIIIth International Symposium on Spermatology
XIIIth International Symposium on Spermatology
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第十三届国际精子学研讨会
DOI：
10.1007/978-3-030-66292-9_21
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Cupples G
中科院分区：
文献类型：
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作者：
Cupples G
精子通过女性生殖道自我推进的能力对自然受精至关重要。尽管如此，人工评估精子活力的范围有限，计算机辅助精子分析（CASA）系统在临床实践中并未常规使用。阻碍CASA系统临床应用的一个重要因素是缺乏将运动测量和生育结果联系起来的证据。为了推进这些技术，我们需要解决样品制备和成像方式的变化，以及当前的运动学参数是否为建立这些联系提供了必要的生理洞察力。在本文中，我们讨论了制备（样品粘度，温度，浓度），采集（室深，帧率，持续时间）和分析如何显著影响CASA结果。为了推进CASA的临床应用，我们概述了获得可在样品和系统之间进行比较的测量值的要求。我们进一步强调如何引入鞭毛跟踪可以形成越来越有见地的诊断的基础；通过将运动学数据与数学模型实验相结合，可以揭示棘手的细节，例如从单个细胞到群体水平的运动代谢需求。
The ability of sperm to self-propel through the female reproductive tract is essential for natural fertilisation. Despite this fact, manual assessments of motility are limited in scope and computer-aided sperm analysis (CASA) systems are not routinely used in clinical practice. One significant factor hindering the clinical use of CASA systems is the lack of evidence linking motility measures and fertility outcomes. To progress these technologies, we need to address the variations in the way samples are prepared and imaged, and whether current kinematic parameters provide the physiological insight necessary for establishing these links. In this manuscript, we discuss how preparation (sample viscosity, temperature, concentration), acquisition (chamber depth, frame rate, duration) and analysis can dramatically affect CASA results. With the aim of advancing the clinical application of CASA, we outline the requirements for obtaining measurements that can be compared between samples and systems. We further highlight how the introduction of flagellar tracking can form the basis of increasingly insightful diagnostics; by combining kinematic data with mathematical modelling experimentally intractable details can be uncovered, such as metabolic requirements of motility from a single cell to the population level.