CELL LINEAGE ANALYSIS IN ASCIDIAN EMBRYOS BY INTRACELLULAR INJECTION OF A TRACER ENZYME .3. UP TO THE TISSUE RESTRICTED STAGE
CELL LINEAGE ANALYSIS IN ASCIDIAN EMBRYOS BY INTRACELLULAR INJECTION OF A TRACER ENZYME .3. UP TO THE TISSUE RESTRICTED STAGE
复制标题
DOI：
10.1016/0012-1606(87)90188-6
复制
发表时间：
1987-06-01
影响因子：
2.7
通讯作者：
NISHIDA, H
中科院分区：
文献类型：
--
作者：
NISHIDA, H
通过使用离子电渗注射法向细胞内注射辣根过氧化物酶，对海鞘类 Halocynthia roretzi 胚胎发生过程中的细胞谱系进行了分析，直到每个卵裂球注定只能成为单一组织类型的阶段（即组织限制阶段）。最初，检查了 64 细胞阶段胚胎的所有卵裂球的发育命运，此后，仅追踪了 64 细胞阶段不受组织限制的那些卵裂球的子卵裂球的命运。卵裂球的发育命运是高度不变的，除了“等价组”的两个候选者（J. Kimble、J. Sulston 和 J. White (1979)。在“Cell Lineage, Stem Cells and Cell Decisionation,”第 59-68 页。Elsevier，阿姆斯特丹/纽约），其中细胞相互作用被认为参与了命运的规范。右侧和左侧a8.25细胞产生耳石和眼孔，右侧和左侧b8.17细胞以互补方式产生脊髓和内胚层链。卵裂球的位置与其衍生物之间不存在固定的关系。细胞命运的大多数限制发生在卵裂早期。产生单一类型组织的卵裂球数量在64细胞阶段为44个，在110细胞阶段为94个。八对卵裂球尚未成为受 110 细胞阶段限制的组织。描述了表皮、神经系统、肌肉、间充质、脊索和内胚层组织的几乎完整的谱系，并构建了囊胚的命运图谱。对于某些组织，原始细胞占据两个不同的区域。还对另一个物种海鞘的胚胎进行了肌肉细胞谱系的补充研究。
Cell lineages during embryogenesis of the ascidian Halocynthia roretzi were analyzed up until the stage where each blastomere was fated to be only a single tissue type (i.e., the tissue restricted stage) by intracellular injection of horseradish peroxidase using the iontophoretic injection method. Initially, the developmental fates of all blastomeres of the 64-cell stage embryo were examined, and thereafter, only the fates of daughter blastomeres of those blastomeres that were not tissue restricted at the 64-cell stage were traced. The developmental fates of blastomeres were highly invariant except for two candidates for "equivalence groups" (J. Kimble, J. Sulston, and J. White (1979). In "Cell Lineage, Stem Cells and Cell Determination," pp. 59-68. Elsevier, Amsterdam/New York), in which cellular interaction is suggested to be involved in the specification of the fates. The right and left a8.25 cells gave rise to the otolith and ocellus, and the right and left b8.17 cells gave rise to the spinal cord and endodermal strand in a complementary manner. No fixed relationship existed between the position of the blastomere and its derivative. Most restrictions of cell fates occurred early in cleavage. The numbers of blastomeres which generated a single type of tissue were 44 at the 64-cell stage and 94 at the 110-cell stage. Eight pairs of blastomeres had not yet become tissue restricted by the 110-cell stage. Almost complete lineages of epidermis, nervous system, muscle, mesenchyme, notochord, and endodermal tissues were described, and a fate map was constructed for the blastula. For certain tissues, the primordial cells occupied two different regions. Supplementary investigations of the lineage of muscle cells were also performed on embryos of another species, Ciona intestinalis.