Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis
Ovarian tissue culture in the presence of VEGF and fetuin stimulates follicle growth and steroidogenesis
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DOI：
10.1530/joe-16-0368
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发表时间：
2017-02-01
影响因子：
4
通讯作者：
Abolhassani, Farid
中科院分区：
文献类型：
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作者：
Asadi, Ebrahim;Najafi, Atefeh;Abolhassani, Farid
卵巢组织冷冻保存和卵泡培养为癌症患者的生育能力保存提供了一种很有前途的技术。该研究旨在评估在添加了VEGFA165和/或胎蛋白的培养基中卵泡参数。将玻璃化加热后的卵巢皮质片随机分为4个培养组，分别为基础培养基（对照）、基础培养基中添加VEGFA165、胎蛋白或两者同时添加。培养6天后，我们评估以下指标：静息、原发和继发卵泡百分率；存活率;类固醇激素的产生；活性氧、脂质过氧化和总抗氧化能力水平；发育和抗氧化基因的表达。在培养液中单独或联合添加VEGFA165可激活静息卵泡，增加生长卵泡的数量。在VEGFA165组中，我们发现17 - β -雌二醇浓度在培养第6天显著增加，黄体酮浓度从培养第4天开始显著增加。在VEGFA165+胎儿素组，17 -雌二醇浓度在培养第4天升高。所有治疗组的BMP15、GDF9和INHB mrna水平均升高。在胚胎素和胚胎素+VEGFA165组中，我们观察到高水平的总抗氧化能力和SOD1和CAT基因的表达，低活性氧和脂质过氧化水平，活卵泡数量增加。综上所述，本研究提供了有用的证据，证明在培养基中添加VEGFA165+胚胎素可以促进原始卵泡的激活和发育，并增加健康的次级卵泡的百分比。
Ovarian tissue cryopreservation together with follicle culture provides a promising technique for fertility preservation in cancer patients. The study aimed to evaluate follicle parameters in a culture medium supplemented with VEGFA165 and/or fetuin. Vitrified-warmed ovarian cortical pieces were divided randomly into four culture groups consisting of basic culture medium (control), and the basic culture medium supplemented with VEGFA165, fetuin or both. After six days of culture, we evaluated the following: percentage of resting, primary and secondary growing follicles; survival rate; steroid hormones production; levels of reactive oxygen species, lipid peroxidation and total antioxidant capacity; and developmental and antioxidant gene expression. The addition of VEGFA165 alone or in combination with fetuin to the culture medium caused resting follicle activation and increased the number of growing follicles. In the VEGFA165 group, we found a significant increase in the concentrations of 17 beta-estradiol at day 6 and progesterone from 4th day of the culture period. In the VEGFA165+fetuin group, the concentration of 17 beta-estradiol rose at day 4 of the culture period. The levels of BMP15, GDF9 and INHB mRNAs were increased in all treated groups. In the fetuin and fetuin+VEGFA165 groups, we observed a high level of total antioxidant capacity and expression of SOD1 and CAT genes, low reactive oxygen species and lipid peroxidation levels and increased number of viable follicles. In conclusion, the present study provides useful evidence that supplementation of culture medium with VEGFA165+fetuin leads to primordial follicle activation and development and increased percentage of healthy secondary growing follicles.