A plasmid partition system of the P1-P7par family from the pMT1 virulence plasmid of Yersinia pestis.
A plasmid partition system of the P1-P7par family from the pMT1 virulence plasmid of Yersinia pestis.
复制标题
来自鼠疫耶尔森氏菌 pMT1 毒力质粒的 P1-P7par 家族的质粒分配系统。
DOI：
10.1128/jb.182.14.3924-3928.2000
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发表时间：
2000
影响因子：
3.2
通讯作者：
Austin,S
中科院分区：
文献类型：
--
作者：
Youngren,B;Radnedge,L;Hu,P;Garcia,E;Austin,S
鼠疫耶尔森氏菌KIM 5毒力质粒pMT 1的全序列显示与大肠杆菌P7质粒前噬菌体的质粒分隔区（par）有同源性。必需基因parA和parS以及下游分配位点基因parS在序列和结构上高度保守。将pMT 1 parSsite和parA-parBoperon分别插入到能在E.杆菌当pMT 1帕拉A和ParB蛋白反式供应时，含有pMT 1 parS的mini-P1载体稳定地维持，表明pMT 1 parS系统在E中是完全功能性的质粒分配。杆菌pMT 1 par系统具有类似于P7 par和P1 par的质粒沉默活性，但比P7 par和P1 par弱。尽管具有高度相似性，尤其是与P7 par的相似性，但它在关键组分的相互作用方面表现出独特的特异性。P7和P1 Par蛋白均不能支持通过pMT 1 parS位点的分配，并且pMT 1 Par蛋白不能支持通过P1 parS或P7 parS的分配。典型的其他分区位点，pMT 1 parSe的额外拷贝对pMT 1 par支持的质粒表现出不相容性。然而，没有观察到种间不亲和性的影响之间的pMT 1 par，P7 par，和P1 par。
The complete sequence of the virulence plasmid pMT1 ofYersinia pestisKIM5 revealed a region homologous to the plasmid partition (par) region of the P7 plasmid prophage ofEscherichia coli. The essential genesparAandparBand the downstream partition site gene,parS, are highly conserved in sequence and organization. The pMT1parSsite and theparA-parBoperon were separately inserted into vectors that could be maintained inE. coli. A mini-P1 vector containing pMT1parSwas stably maintained when the pMT1 ParA and ParB proteins were supplied intrans, showing that the pMT1parsystem is fully functional for plasmid partition inE. coli. The pMT1parsystem exerted a plasmid silencing activity similar to, but weaker than those of P7parand P1par. In spite of the high degree of similarity, especially to P7par, it showed unique specificities with respect to the interactions of key components. Neither the P7 nor P1 Par proteins could support partition via the pMT1parSsite, and the pMT1 Par proteins failed to support partition with P1parSor P7parS. Typical of other partition sites, supernumerary copies of pMT1parSexerted incompatibility toward plasmids supported by pMT1par. However, no interspecies incompatibility effect was observed between pMT1par, P7par, and P1par.