PSVII-25 Grazing-induced transcriptomic changes in bovine biceps femoris muscle, subcutaneous fat, and liver mRNAs and plasma exosome microRNAs.
PSVII-25 Grazing-induced transcriptomic changes in bovine biceps femoris muscle, subcutaneous fat, and liver mRNAs and plasma exosome microRNAs.
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PSVII-25 放牧诱导的牛股二头肌、皮下脂肪、肝脏 mRNA 和血浆外泌体 microRNA 的转录组变化。
DOI：
10.1093/jas/sky404.783
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发表时间：
2018
影响因子：
3.3
通讯作者：
Hojito M
中科院分区：
文献类型：
--
作者：
Muroya S;Ogasawara H;Nohara K;Oe M;Ojima K;Hojito M
放牧改变了牛的骨骼肌生理特性。我们假设循环microRNA （c-miRNA）水平的变化归因于脂肪和肝脏组织外泌体miRNA的释放和/或摄取。在本研究中，为了确定放牧诱导的这些组织的mRNA和miRNA转录组变化，我们对日本短刺牛的股二头肌（BF）、皮下脂肪（ScF）和肝脏组织进行了微阵列分析。放牧组和圈养组各分配3头20 ~ 22月龄阉牛；放牧阉牛从5月中旬开始在牧场上喂养3个月，其他阉牛则被安置在一个自由栏的谷仓里，每天早上收割草。放牧期3个月采集标本，如有实体组织，行手术活检。血浆外泌体（pEX）由血液样品制备，采用超离心。从组织中制备总rna，并在每个组织中汇总，最后应用于mRNA和miRNA表达微阵列分析。变异基因数以BF最多，增加基因52个，减少基因1088个。另一方面，与饲养组相比，增加（INC）和减少（DEC） 2倍以上的基因数量在ScF中最多，在肝脏中最少。特别是在ScF中，我们观察到169个INC基因和249个DEC基因，表明在放牧牛ScF中，DEC基因比INC基因占优势。利用INC和DEC基因列表，我们进行了基因本体分析，并从ScF中的DEC基因中提取了术语“细胞外泌体”，作为与放牧牛ScF变化相关的术语。这与pEX中减少的mirna数量多于增加的mirna数量相一致。这些结果表明放牧诱导的c- mirna变化与牛ScF转录组有关。
Grazing alters skeletal muscle physiological properties in cattle. We hypothesized that changes in circulating microRNA (c-miRNA) levels are attributed to release and/or uptake of exosomal miRNA at adipose and liver tissues. In this study, to determine grazing-induced mRNA and miRNA transcriptomic changes at those tissues, we conducted microarray analyses in biceps femoris muscle (BF), subcutaneous fat (ScF), and liver tissues of Japanese Shorthorn steers. Three steers (aged 20–22 mo) were allocated to each of grazing and housed groups; grazing steers were fed on pasture for 3 months from middle of May, when the other steers were housed and fed in a free-stall barn with grass harvested every morning. The samples were collected at 3 mo of grazing period, with surgical biopsy in case of solid tissues. Plasma exosomes (pEX) were prepared from the blood samples, using ultracentrifugation. Total RNAs were prepared from the tissues and pooled within each tissue, and finally applied to both mRNA and miRNA expression microarray analyses. The number of changed genes was the highest in BF (52 increased and 1088 decreased genes). On the other hand, the number of genes showing more than 2-fold increase (INC) and decrease (DEC) compared to the housed was the highest in ScF and the lowest in liver. Especially in ScF, we observed 169 INC and 249 DEC genes, indicating dominance of DEC genes over INC genes in ScF of the grazing steers. Using the list of INC and DEC genes, we conducted gene ontology analyses and extracted the term ‘extracellular exosome’ from the DEC genes in ScF, as a term associated with changes in ScF of the grazing steers. This was coincident with greater number of the decreased miRNAs than the increased in pEX. These results suggest an association of grazing-induced changes in c-miRNAs with ScF transcriptome in the steers.