Modulation of host cell membrane fluidity: a novel mechanism for preventing bacterial adhesion
Modulation of host cell membrane fluidity: a novel mechanism for preventing bacterial adhesion
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DOI：
10.1152/ajpgi.1999.277.1.g201
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发表时间：
1999-07-01
影响因子：
4.5
通讯作者：
Sherman, PM
中科院分区：
文献类型：
--
作者：
Ismaili, A;Meddings, JB;Sherman, PM
肠道致病菌与宿主粘膜表面的粘附是腹泻发病的关键步骤。我们研究了改变真核细胞的物理性质对细菌粘附的影响，使用一系列三种结构不同的膜流化器和几种大肠杆菌作为试验菌株。采用1-（4-三甲基氨基苯基）-6-苯基-1己三烯探针，采用稳态荧光各向异性法测定细胞膜脂质流动性。随着膜流动性的增加，细菌粘附的抑制呈剂量依赖性和可逆性。时间过程实验表明，在细菌粘附的早期阶段增加膜的流动性是抑制附着的必要条件。这些流化器均不影响真核细胞或原核细胞的活力。这些发现首次证明，上皮细胞的质膜物理性质的改变可以阻止微生物的粘附。这也表明，改变宿主细胞的膜特性可能为在体内感染过程中预防细菌粘附的新策略奠定基础。
Adhesion of bacterial enteropathogens to host mucosal surfaces is a critical primary step in the pathogenesis of diarrheal disease. We investigated the effects of altering the physical properties of eukaryotic cells on bacterial adhesion with the use of a series of three structurally dissimilar membrane fluidizers and several Escherichia coli as test strains. Lipid fluidity of the cell plasma membrane was measured by steady-state fluorescence anisotropy employing the probe 1-(4-trimethylammoniumphenyl)-6-phenyl-1 hexatriene. There was a dose-dependent and reversible inhibition of bacterial adhesion with increasing membrane fluidity. Time course experiments indicated that increasing membrane fluidity during the early stages of bacterial adhesion was essential for inhibition of attachment. None of the fluidizers affected the viability of either eukaryotic or prokaryotic cells. These findings demonstrate, for the first time, that changes in plasma membrane physical properties of epithelial cells can prevent microbial adhesion. This also suggests that altering the membrane properties of host cells could form a basis for novel strategies to prevent bacterial adhesion during infection in vivo.