Luteolibacter arcticus sp. nov., isolated from high Arctic tundra soil, and emended description of the genus Luteolibacter.
Luteolibacter arcticus sp. nov., isolated from high Arctic tundra soil, and emended description of the genus Luteolibacter.
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DOI：
10.1099/ijs.0.000202
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发表时间：
2015-06
影响因子：
2.8
通讯作者：
Myongchol Kim;Sehong Pak;S. Rim;Lvzhi Ren;F. Jiang;Xulu Chang;Ping Liu;Yumin Zhang;C. Fang;Congyi Zheng;Fang Peng
中科院分区：
文献类型：
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作者：
Myongchol Kim;Sehong Pak;S. Rim;Lvzhi Ren;F. Jiang;Xulu Chang;Ping Liu;Yumin Zhang;C. Fang;Congyi Zheng;Fang Peng
从挪威斯瓦尔巴群岛（78°N） Ny-Ålesund附近的冻土带土壤中分离出一种淡黄色，革兰氏反应阴性，无运动的需氧细菌，命名为MC 3726T。生长条件为4-37℃（最适25-30℃）和pH 5.0-9.0（最适pH 8.0）。基于16S rRNA基因序列的系统发育分析表明，菌株mc3726t属于Verrucomicrobiaceae木犀草杆菌属（Luteolibacter）。该菌株的16S rRNA基因序列与cuticulihirudinis E100T、pohnpeiensis A4T-83T和yonseiensis EBTL01T的相似性分别为93.18%、92.54%和92.44%。菌株mc3726t细胞壁以中二氨基戊酸为诊断氨基酸。菌株MC 3726T细胞脂肪酸主要为iso-C14:0（38.28%）、C16:0（15.89%）、C16:1ω9c（14.24%）、iso-C16:0（10.42%）和前iso- c15:0(5.75%)，呼吸类醌主要为MK-9和MK-10，极性脂质主要为磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰甘油和二磷脂酰甘油。DNA G+C含量为60.7 mol %。根据表型、化学分类和系统发育数据，菌株mc3726t被认为是Luteolibacter属的一个新种，并建议将其命名为Luteolibacter arcticus sp. 11。型应变为MC 3726T （= CCTCC AB 2014275T = LMG 28638T）。还提供了对Luteolibacter属的修订描述，以及对cuticulihirudinis， Luteolibacter yonseiensis和Luteolibacter pohnpeiensis的修订描述。
A pale yellow, Gram-reaction-negative, non-motile, aerobic bacterium, designated MC 3726T, was isolated from a tundra soil near Ny-Ålesund, Svalbard Archipelago, Norway (78 °N). Growth occurred at 4-37 °C (optimum 25-30 °C) and at pH 5.0-9.0 (optimum pH 8.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MC 3726T belonged to the genus Luteolibacter in the family Verrucomicrobiaceae. The 16S rRNA gene sequence of this strain showed 93.18, 92.54 and 92.44 % similarity to those of Luteolibacter cuticulihirudinis E100T, Luteolibacter pohnpeiensis A4T-83T and Luteolibacter yonseiensis EBTL01T, respectively. The cell wall of strain MC 3726T contained meso-diaminopimelic acid as the diagnostic amino acid. Strain MC 3726T contained iso-C14:0 (38.28 %), C16:0 (15.89 %), C16:1ω9c (14.24 %), iso-C16:0 (10.42 %) and anteiso-C15:0 (5.75 %) as the predominant cellular fatty acids, MK-9 and MK-10 as the major respiratory quinones, and phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the main polar lipids. The DNA G+C content was 60.7 mol %. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain MC 3726T is considered to represent a novel species of the genus Luteolibacter, for which the name Luteolibacter arcticus sp. nov. is proposed. The type strain is MC 3726T ( = CCTCC AB 2014275T = LMG 28638T). An emended description of the genus Luteolibacter is also provided, along with emended descriptions of Luteolibacter cuticulihirudinis, Luteolibacter yonseiensis and Luteolibacter pohnpeiensis.