Toward a long-range map of human chromosomal band 22q11.
Toward a long-range map of human chromosomal band 22q11.
复制标题
绘制人类染色体带 22q11 的远程图谱。
DOI：
10.1016/0888-7543(89)90079-7
复制
发表时间：
1989
期刊：
影响因子：
4.4
通讯作者：
Emanuel,BS
中科院分区：
文献类型：
--
作者：
McDermid,HE;Budarf,ML;Emanuel,BS
人类染色体22q11带参与了大量的染色体重排。该区域的远程分子图谱将允许更精确地定位这些重排的各种断点。为了实现这一目标，我们构建了一个基因组DNA文库，可以分离出与NotI位点直接相邻的DNA克隆。之所以选择NotI，是因为它是一种在人类基因组中很少消化的限制性内切酶。这个文库中使用的基因组DNA来自人/仓鼠杂交细胞系，该细胞系的22号染色体是唯一可见的人类染色体。利用体细胞杂交定位板分离了两个克隆，并将其定位于22q11的不同区域。使用NotI和其他不常用的切割酶制作了这两个克隆中每个克隆NotI位点两侧的长距离限制性图谱。分析的两个NotI位点都位于HTF岛，这些区域通常与基因的5‘端相关。因此，22q11的NotI图谱也可能有助于克隆未发现的基因，为该地区复制/缺失综合征的研究提供了一个起点。
Human chromosome band 22q11 is involved in numerous chromosomal rearrangements. A long-range molecular map of this region would allow the more precise localization of the various breakpoints of these rearrangements. Toward this goal we have constructed a genomic DNA library that allows the isolation of DNA clones that are directly adjacent toNotI sites.NotI was chosen because it is a restriction enzyme that digests infrequently in the human genome. The genomic DNA used in this library was from a human/hamster hybrid cell line that has a chromosome 22 as the only visible human chromosome. Two clones were isolated and mapped to different regions of 22q11 using a somatic cell hybrid mapping panel. A long-range restriction map flanking theNotI site of each of these two clones was produced usingNotI and other infrequently cutting enzymes. BothNotI sites analyzed were located in HTF islands, regions often associated with the 5′ end of genes. Thus, theNotI map of 22q11 may also aid in the cloning of undiscovered genes, giving a starting point for the study of duplication/deficiency syndromes of the region.