A dietary pattern associated with LINE-1 methylation alters the risk of developing cervical intraepithelial neoplasia.
A dietary pattern associated with LINE-1 methylation alters the risk of developing cervical intraepithelial neoplasia.
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DOI：
10.1158/1940-6207.capr-11-0387
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Partridge EE
中科院分区：
文献类型：
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作者：
Piyathilake CJ;Badiga S;Kabagambe EK;Azuero A;Alvarez RD;Johanning GL;Partridge EE
很少有研究探讨饮食模式 (DP) 与发生癌前病变的风险以及与此类 DP 相关的生物标志物之间的关系。本研究的目的是鉴定与较高级别宫颈上皮内瘤变 (CIN 2+) 相关的 DP，并确定这些 DP 是否与外周血单核细胞 (PBMC) 长散布核苷酸元件 (L1) 的 DNA 甲基化程度相关，这是一种与发生 CIN 2+ 风险相关的生物标志物。研究人群包括 319 名育龄妇女。 DP 通过因子分析得出。通过焦磷酸测序评估 PBMC L1 甲基化程度。 Logistic 回归模型用于评估 DP 与 CIN 2+ 之间的关联。使用类似的模型来评估无 CIN 2+ 的女性中 DP 与 PBMC L1 甲基化程度之间的关联。与 DP 最健康的女性相比，DP 最不健康的女性被诊断为 CIN 2+ 的可能性是 3.5 倍（OR=3.5；95% CI，1.2–10.1；P=0.02）。与 DP 最不健康的女性相比，DP 最健康、有发生 CIN 2+ 风险的女性 PBMC L1 甲基化水平较高的可能性是 3.3 倍（OR=3.3；95% CI，1.0-10.6；P=0.04）。我们的研究结果表明，通过改善 DP 可以降低 HPV 相关的发生 CIN 2+ 的风险。 PBMC L1 甲基化程度可作为监测降低 CIN 2+ 风险所需饮食调整有效性的生物标志物。
There is a paucity of research examining the relationships between dietary patterns (DPs) and risk of developing pre-cancerous lesions as well as biomarkers associated with such DPs. The purpose of the current study was to identify DPs that are associated with higher grades of cervical intraepithelial neoplasia (CIN 2+) and to determine whether these DPs are associated with the degree of DNA methylation in the long interspersed nucleotide elements (L1s) of peripheral blood mononuclear cells (PBMCs), a biomarker associated with risk of developing CIN 2+. Study population consisted of 319 child-bearing age women. DPs were derived by factor analysis. The degree of PBMC L1 methylation was assessed by pyrosequencing. Logistic regression models were used to evaluate the associations between DPs and CIN 2+. Similar models were used to evaluate the association between DPs and degree of PBMC L1 methylation in women free of CIN 2+. Women with the unhealthiest DP were 3.5 times more likely to be diagnosed with CIN 2+ compared to women with the healthiest DP (OR=3.5; 95% CI, 1.2–10.1; P=0.02). Women at risk for developing CIN 2+ with the healthiest DP were 3.3 times more likely to have higher PBMC L1 methylation compared to women with the unhealthiest DP (OR=3.3; 95% CI, 1.0–10.6; P=0.04). Our findings suggest that HPV associated risk of developing CIN 2+ may be reduced by improving DPs. The degree of PBMC L1 methylation may serve as a biomarker for monitoring the effectiveness of dietary modifications needed for reducing the risk of CIN 2+.