Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don]
Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle [Catharanthus roseus (L.) G. Don]
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DOI：
10.1007/s11738-010-0631-6
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发表时间：
2011-05-01
影响因子：
2.6
通讯作者：
Moinuddin
中科院分区：
文献类型：
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作者：
Idrees, Mohd;Naeem, M.;Moinuddin
通过盆栽试验，探讨叶面喷施水杨酸（SA）能否成功改善盐胁迫对长春花的不利影响。三十天龄的植物提供有对照； 0 mM 氯化钠 + 10(-5) M SA (T-1); 50 mM 氯化钠 + 0 SA (T-2); 100 mM 氯化钠 + 0 SA (T-3)； 150 mM 氯化钠 + 0 SA (T-4)； 50 mM 氯化钠 + 10(-5) M SA (T-5)； 100 mM 氯化钠 + 10(-5) M SA (T-6)； 150 mM 氯化钠 + 10(-5) M SA (T-7)。播种后 90 天对植物进行取样，以评估 SA 对胁迫和未胁迫植物的影响。盐胁迫显着降低了生长特性，包括株高、叶面积指数、芽和根鲜重、芽和根干重。增加氯化钠浓度导致光合参数以及硝酸还原酶和碳酸酐酶活性逐渐下降。在氯化钠处理的植物中，抗坏血酸、总生物碱和抗氧化剂酶超氧化物歧化酶、过氧化氢酶和过氧化物酶也有所下降。遭受氯化钠胁迫的植物表现出电解质渗漏和脯氨酸含量显着增加。叶面喷施SA(10(-5)M)可减轻盐分对植物生长的损害作用，加速生长过程的恢复。它不仅改善了生长参数，还逆转了盐度的影响。在未胁迫和胁迫植物中施用SA均提高了总生物碱含量。 SA处理胁迫植物叶片中记录的总生物碱含量最高水平为11.1%。叶面喷洒SA可以提高100 M NaCl处理植物中长春新碱(14.0%)和长春花碱(14.6%)的含量，从而克服了盐分的不利影响。
A pot experiment was conducted to find out whether the foliar spray of salicylic acid (SA) could successfully ameliorate the adverse effects of salinity stress on periwinkle. Thirty-day-old plants were supplied with Control; 0 mM NaCl + 10(-5) M SA (T-1); 50 mM NaCl + 0 SA (T-2); 100 mM NaCl + 0 SA (T-3); 150 mM NaCl + 0 SA (T-4); 50 mM NaCl + 10(-5) M SA (T-5); 100 mM NaCl + 10(-5) M SA (T-6); 150 mM NaCl + 10(-5) M SA (T-7). The plants were sampled 90 days after sowing to assess the effect of SA on stressed and unstressed plants. Salt stress significantly reduced the growth attributes including plant height, leaf-area index, shoot and root fresh weights, shoot and root dry weights. Increasing NaCl concentrations led to a gradual decrease in photosynthetic parameters and activities of nitrate reductase and carbonic anhydrase. Ascorbic acid, total alkaloids and antioxidants enzymes superoxide dismutase, catalase and peroxidase also declined in NaCl-treated plants. The plants, undergoing NaCl stress, exhibited a significant increase in electrolyte leakage and proline content. Foliar application of SA (10(-5) M) reduced the damaging effect of salinity on plant growth and accelerated the restoration of growth processes. It not only improved the growth parameters but also reversed the effects of salinity. Total alkaloid content was improved by SA application both in unstressed and stressed plants. The highest level of total alkaloid content recorded in leaves of SA-treated stressed plants was 11.1%. Foliar spray of SA overcame the adverse effect of salinity by improving the content of vincristine (14.0%) and vinblastine (14.6%) in plants treated with 100 M NaCl.