Changes in articular cartilage and subchondral bone histomorphometry in osteoarthritic knee joints in humans
Changes in articular cartilage and subchondral bone histomorphometry in osteoarthritic knee joints in humans
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DOI：
10.1016/s8756-3282(02)00982-1
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发表时间：
2003-03-01
期刊：
影响因子：
4.1
通讯作者：
Maric, I
中科院分区：
文献类型：
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作者：
Bobinac, D;Spanjol, J;Maric, I
在这项研究中，我们已经研究了关节软骨的组织学和组织化学变化与软骨下骨的骨参数之间的相关性。目的是阐明宏观正常软骨关节和骨关节炎（OA）关节中不同深度软骨下骨内骨参数变化的模式。在全膝关节置换术期间，由于严重OA，从患者中取出10个胫骨平台。并与10组无骨关节病史的尸检受试者的胫骨髁进行比较。从髁突的前、后、外、内四个区域取出圆柱形软骨-骨样本，用于软骨评估（Mankin评分）和软骨下骨组织形态计量学。测量骨体积（BV/TV）、骨小梁厚度（Tb.Th）、骨小梁数量（Tb.N）和骨小梁间距（Tb.S）。我们的研究表明，OA组的软骨下骨的骨体积（54.1 ± 10.6%）显著高于对照组（37.8 ± 8.1%）（P < 0.01）。OA软骨下骨的骨小梁参数显示骨小梁数量较对照组少，分布稀疏，骨小梁厚度较对照组厚（P < 0.05）。对照组的内侧和外侧髁没有显著差异，而OA组的内侧髁显示出骨体积的高增加（62.8 +/- 13.3），并且与同组的外侧髁相比，骨小梁参数连续不同。此外，研究表明，对照组和OA组的两个髁突之间的骨参数存在区域差异（前部、后部、内部和外部）。比较三个不同阶段的关节软骨退变（Mankin评分）的骨参数表明，软骨退变程度越高，软骨下骨结构的变化越明显。此外，我们发现软骨退化的进展导致骨参数的变化，影响更深层次的软骨下骨。根据这些结果，可以建议，软骨下骨的组织形态计量学参数的变化是继发于软骨损伤，并进行更深的软骨下骨软骨退化的增加。(C)2003 Elsevier Science（美国）。All rights reserved.
In this study, we have examined the correlation between the histological and histochemical changes of articular cartilage and bone parameters of the underlying subchondral bone. The aim was to elucidate patterns of bone parameter changes within different depths of subchondral bone in the joints with macroscopically normal cartilage and in joints with osteoarthritis (OA). Ten tibial plateaus were taken from patients during total knee replacement surgery due to severe OA. They were compared with 10 sets of tibial condyles obtained from autopsy subjects with no history of bone or joint disease. The cylindrical cartilage-bone samples were taken out from the anterior, posterior, external, and internal areas of the condyles for cartilage assessment (Mankin score) and subchondral bone histomorphometry. Four histomorphometric parameters were measured: bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.S). Our study showed that subchondral bone from the OA group had significantly higher bone volume (54.1 +/- 10.6%) than control group (37.8 +/- 8.1%) (P < 0.01). In addition, trabecular parameters from the OA subchondral bone showed a smaller number of sparsely distributed and thicker trabecules than in control group (P < 0.05). Medial and lateral condyle from the control group did not differ significantly, while medial condyle from OA group showed a high increase of bone volume (62.8 +/- 13.3) and consecutively different trabecular parameters when compared with the lateral condyle from the same group. Also, it was shown that there are regional differences (anterior, posterior, internal, and external) in bone parameters between both condyles within both, control and OA groups. Comparison of bone parameters from three different stage of articular cartilage degeneration (Mankin score) showed that higher degree of cartilage degeneration is followed by significant changes in subchondral bone architecture. Furthermore, we have found that progression of cartilage degeneration leads to changes in bone parameters which affected deeper levels of subchondral bone. According to these results, it can be suggested that changes in histomorphometric parameters of subchondral bone are secondary to cartilage damage and proceed deeper into subchondral bone with increasing cartilage degeneration. (C) 2003 Elsevier Science (USA). All rights reserved.