Untargeted Metabolic Quantitative Trait Loci Analyses Reveal a Relationship between Primary Metabolism and Potato Tuber Quality
Untargeted Metabolic Quantitative Trait Loci Analyses Reveal a Relationship between Primary Metabolism and Potato Tuber Quality
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DOI：
10.1104/pp.111.188441
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发表时间：
2012-03-01
期刊：
影响因子：
7.4
通讯作者：
Keurentjes, Joost J. B.
中科院分区：
文献类型：
--
作者：
Carreno-Quintero, Natalia;Acharjee, Animesh;Keurentjes, Joost J. B.
转录组学、代谢组学和蛋白质组学以及基因型分析等组学技术的最新进展使得能够对非模式物种中以分子表型为代表的复杂性状的遗传学进行剖析。为了确定马铃薯（Solanum tuberosum）初级代谢变化的遗传因素，我们使用气相色谱-飞行时间质谱法分析了二倍体马铃薯作图群体中的初级代谢物含量，该群体源自马铃薯和野生近缘种之间的杂交。总共检测到 139 种极性代谢物，其中我们鉴定了约 72% 检测到的化合物的代谢物数量性状位点。为了深入了解代谢性状和经典表型性状之间的关系，我们还分析了它们之间的统计关联。通过数量性状基因座重合和统计学习方法的应用对遗传信息进行组合分析，提供了与影响复杂表型性状的代谢网络变化相关的推定指标的信息。
Recent advances in -omics technologies such as transcriptomics, metabolomics, and proteomics along with genotypic profiling have permitted dissection of the genetics of complex traits represented by molecular phenotypes in nonmodel species. To identify the genetic factors underlying variation in primary metabolism in potato (Solanum tuberosum), we have profiled primary metabolite content in a diploid potato mapping population, derived from crosses between S. tuberosum and wild relatives, using gas chromatography-time of flight-mass spectrometry. In total, 139 polar metabolites were detected, of which we identified metabolite quantitative trait loci for approximately 72% of the detected compounds. In order to obtain an insight into the relationships between metabolic traits and classical phenotypic traits, we also analyzed statistical associations between them. The combined analysis of genetic information through quantitative trait locus coincidence and the application of statistical learning methods provide information on putative indicators associated with the alterations in metabolic networks that affect complex phenotypic traits.