Zinc finger protein 5 (ZFP5) associates with ethylene signaling to regulate the phosphate and potassium deficiency-induced root hair development in Arabidopsis
Zinc finger protein 5 (ZFP5) associates with ethylene signaling to regulate the phosphate and potassium deficiency-induced root hair development in Arabidopsis
复制标题
锌指蛋白 5 (ZFP5) 与乙烯信号传导相关，调节拟南芥中磷酸盐和钾缺乏诱导的根毛发育
DOI：
10.1007/s11103-019-00937-4
复制
发表时间：
2020-01-01
影响因子：
5.1
通讯作者：
Gan, Yinbo
中科院分区：
文献类型：
--
作者：
Huang, Linli;Jiang, Qining;Gan, Yinbo
锌指蛋白转录因子ZFP5主要通过激活EIN2在拟南芥中的表达，正向调节根毛的伸长以响应磷和钾的缺乏。磷(PI)和钾(K+)是植物生长发育所需的主要营养物质，植物通过代谢和形态变化来响应低营养条件。C2H2转录因子ZFP5是拟南芥毛和根毛发育的关键调控因子。然而，它在营养剥夺条件下调节根毛发育的作用尚不清楚。在此，我们发现缺磷和缺钾不能使Zfp5突变体和ZFP5 RNAi系的短根毛表型恢复到野生型水平。剥夺这两种营养物质还诱导了ZFP5的表达和乙烯报告基因PEBS：GUS的活性。在缺磷和缺钾条件下，Ein2-1和Ein3-1的根毛长度较野生型显著缩短，支持乙烯参与根毛伸长。此外，施加1-氨基环丙烷-1-羧酸(ACC)显著提高ZFP-5的表达水平，而施加2-氨基乙氧基乙烯基甘氨酸(AVG)则相反。进一步的实验表明，ZFP5主要调控乙烯不敏感2(EIN2)的转录，通过乙烯信号转导来控制缺糖介导的根毛发育。综上所述，这些结果表明，在拟南芥中，ZFP5通过与乙烯信号相互作用来调节根毛伸长，主要是通过调节EIN2的表达来响应磷和钾的缺乏。
Zinc finger protein transcription factor ZFP5 positively regulates root hair elongation in response to Pi and potassium deficiency by mainly activating the expression of EIN2 in Arabidopsis. Phosphate (Pi) and potassium (K+) are major plant nutrients required for plant growth and development, and plants respond to low-nutrient conditions via metabolic and morphology changes. The C2H2 transcription factor ZFP5 is a key regulator of trichome and root hair development in Arabidopsis. However, its role in regulating root hair development under nutrient deprivations remains unknown. Here, we show that Pi and potassium deficiency could not restore the short root hair phenotype of zfp5 mutant and ZFP5 RNAi lines to wild type level. The deprivation of either of these nutrients also induced the expression of ZFP5 and the activity of an ethylene reporter, pEBS:GUS. The significant reduction of root hair length in ein2-1 and ein3-1 as compared to wild-type under Pi and potassium deficiency supports the involvement of ethylene in root hair elongation. Furthermore, the application of 1-aminocyclopropane-1-carboxylic acid (ACC) significantly enhanced the expression level of ZFP5 while the application of 2-aminoethoxyvinyl glycine (AVG) had the opposite effect when either Pi or potassium was deprived. Further experiments reveal that ZFP5 mainly regulates transcription of ETHYLENE INSENSITIVE 2 (EIN2) to control deficiency-mediated root hair development through ethylene signaling. Generally, these results suggest that ZFP5 regulates root hair elongation by interacting with ethylene signaling mainly through regulates the expression of EIN2 in response to Pi and potassium deficiency in Arabidopsis.