The monomeric clathrin assembly protein, AP180, regulates contractile vacuole size in Dictyostelium discoideum.
The monomeric clathrin assembly protein, AP180, regulates contractile vacuole size in Dictyostelium discoideum.
复制标题
单体网格蛋白组装蛋白 AP180 调节盘基网柄菌中的收缩液泡大小。
DOI：
10.1091/mbc.e06-06-0531
复制
发表时间：
2006
影响因子：
3.3
通讯作者：
O'Halloran,TheresaJ
中科院分区：
文献类型：
--
作者：
Stavrou,Irene;O'Halloran,TheresaJ
AP180是网格蛋白的许多装配蛋白和衔接子之一，刺激网格蛋白晶格在膜上的装配，但其对网格蛋白功能的独特贡献仍然难以捉摸。在这项研究中，我们确定了盘基网柄藻的接头蛋白AP 180的直系同源物，并表征了携带该基因缺失的突变株。GFP标记的AP 180的成像显示，它定位于质膜、收缩泡和细胞质的点，并与网格蛋白相关。AP180无效细胞没有显示网格蛋白突变体的缺陷特征，并继续定位网格蛋白斑点在其质膜上和细胞质内。然而，与网格蛋白突变体一样，AP 180突变体对蛋白酶敏感。当浸入水中时，AP 180无效细胞形成异常大的收缩空泡。此外，在AP 80 null细胞中收缩空泡的扩张和收缩周期是野生型细胞的两倍长。总之，我们的研究结果表明，AP 180起着独特的作用，作为一个调节器的收缩泡的形态和活动inDictyosteopathy。
AP180, one of many assembly proteins and adaptors for clathrin, stimulates the assembly of clathrin lattices on membranes, but its unique contribution to clathrin function remains elusive. In this study we identified theDictyostelium discoideumortholog of the adaptor protein AP180 and characterized a mutant strain carrying a deletion in this gene. Imaging GFP-labeled AP180 showed that it localized to punctae at the plasma membrane, the contractile vacuole, and the cytoplasm and associated with clathrin. AP180 null cells did not display defects characteristic of clathrin mutants and continued to localize clathrin punctae on their plasma membrane and within the cytoplasm. However, like clathrin mutants, AP180 mutants, were osmosensitive. When immersed in water, AP180 null cells formed abnormally large contractile vacuoles. Furthermore, the cycle of expansion and contraction for contractile vacuoles in AP80 null cells was twice as long as that of wild-type cells. Taken together, our results suggest that AP180 plays a unique role as a regulator of contractile vacuole morphology and activity inDictyostelium.