Identification of three novel SNPs and association with carcass traits in porcine TNNI1 and TNNI2
Identification of three novel SNPs and association with carcass traits in porcine TNNI1 and TNNI2
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DOI：
10.1007/s11033-010-0010-9
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Z. Xu;H. Yang;Y. Xiong;C. Deng;Fenge Li;M. Lei;Bo Zuo
中科院分区：
文献类型：
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作者：
Z. Xu;H. Yang;Y. Xiong;C. Deng;Fenge Li;M. Lei;Bo Zuo
本研究采用Xba I、Msp I和Sma I限制性内切酶对TNNI 1和TNNI 2进行了PCR-RFLP分析，发现了两个新的SNP位点（EU 743939：g.5174T>C in intron 4和EU 743939：g.8350C>A in intron 7）和一个SNP位点（EU 696779：g.1167C>T in intron 3）。对10个中国地方猪种和3个西方商品猪种的3个新SNPs的等位基因频率进行了测定。以大白色×梅山F2猪群体为研究对象，对SNPs与胴体性状进行关联分析。TNNI 1基因中两个SNPs（g.5174T>C和g.8350C>A）的连锁对脂肪率有显著影响。此外，g.5174T>C基因多态性还与皮肤百分比（P< 0.05）、肩部脂肪厚度（P< 0.05）和第6、7肋背膘厚度（P< 0.05）显著相关。TNNI 2基因g.1167C>T多态性对肥育率（P< 0.01）、瘦肉率（P< 0.05）、狮眼面积（P <0.05）、胸腰背膘厚（P< 0.01）和平均背膘厚（P< 0.05）有显著影响。
In this study, two novel SNPs (EU743939:g.5174T>C in intron 4 and EU743939:g.8350C>A in intron 7) inTNNI1and one SNP (EU696779:g.1167C>T in intron 3) inTNNI2were identified by PCR–RFLP (PCR restriction fragment length polymorphism) usingXbaI,MspI andSmaI restriction enzyme, respectively. The allele frequencies of three novel SNPs were determined in the genetically diverse pig breeds including ten Chinese indigenous pigs and three Western commercial pig breeds. Association analysis of the SNPs with the carcass traits were conducted in a Large White × Meishan F2pig population. The linkage of two SNPs (g.5174T>C and g.8350C>A) inTNNI1gene had significant effect on fat percentage. Besides these, the g.5174T>C polymorphism was also significantly associated with skin percentage (P< 0.05), shoulder fat thickness (P< 0.05) and backfat thickness between sixth and seventh ribs (P< 0.05). The significant effects of g.1167C>T polymorphism inTNNI2gene on fat percentage (P< 0.01), lean meat percentage (P< 0.05), lion eye area (P< 0.05), thorax–waist backfat thickness (P< 0.01) and average backfat thickness (P< 0.05) were also found.