Yolk testosterone varies with sex in eggs of the lizard, Anolis carolinensis.
Yolk testosterone varies with sex in eggs of the lizard, Anolis carolinensis.
复制标题
蜥蜴 Anolis carolinensis 卵中的卵黄睾酮随性别的不同而变化。
DOI：
10.1002/jez.a.10225
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Wade,Juli
中科院分区：
文献类型：
--
作者：
Lovern,MatthewB;Wade,Juli
在绿色变色龙（Anolis carolinensis）中，一种具有基因型性别决定的蜥蜴，产卵时产雄卵的卵黄睾酮（T）浓度高于产雌卵，但来源和潜在影响从以前的研究中尚不清楚。如果蛋黄T水平在产卵前也是性别特异性的，在此期间胚胎类固醇合成是不可能的，这将强烈表明蛋黄T的差异是母系来源的。我们从麻醉的雌性输卵管内的蛋壳中收集蛋黄样品，然后让这些雌性自然产卵。卵孵育至孵化，以确定性别形态学，并通过放射免疫测定法分析蛋黄T浓度。就像刚产下卵后的情况一样，雄性输卵管卵的卵黄T高于雌性输卵管卵。据我们所知，这是最早的性别差异报告的任何蛋黄类固醇。我们认为，母体来源的卵黄T水平可以通过差异影响男性和女性诱导精子来影响性别，因为受精发生在卵黄沉积和排卵之后，而卵子在输卵管中。我们的研究结果，以及越来越多的研究结果表明，激素和脊椎动物性别决定之间的关系可能比一般认识的更广泛。Zool.295A：206-210，2003.© 2003 Wiley利斯公司
In the green anole (Anolis carolinensis), a lizard with genotypic sex determination, yolk testosterone (T) concentration is greater in male‐producing than female‐producing eggs at oviposition, but the source and potential effects were not clear from previous studies. If yolk T levels are also sex‐specific before eggs are laid, a period during which embryonic steroidogenesis is unlikely, it would strongly suggest that the difference in yolk T is maternally derived. We collected yolk samples from eggs shelling within the oviducts of anesthetized females, and then allowed these females to lay the eggs naturally. Eggs were incubated to hatching to determine sex morphologically, and yolk T concentrations were analyzed by radioimmunoassay. As is the case just after they are laid, yolk T is higher in male than female oviductal eggs. To our knowledge, this is the earliest sex difference reported for any yolk steroid. We suggest that maternally derived yolk T levels could influence sex by differentially affecting male‐ and female‐inducing sperm, because fertilization occurs after yolk deposition and ovulation, while the egg is in the oviduct. Our results, together with those of an increasing number of studies, suggest that a relationship between hormones and vertebrate sex determination may be more widespread than generally appreciated.J. Exp. Zool. 295A:206–210, 2003. © 2003 Wiley‐Liss, Inc.