Tissue response and Msx1 expression after human fetal digit tip amputation in vitro
Tissue response and Msx1 expression after human fetal digit tip amputation in vitro
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DOI：
10.1111/j.1743-6109.2006.00139.x
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发表时间：
2006-07-01
影响因子：
2.9
通讯作者：
Clark, John M.
中科院分区：
文献类型：
--
作者：
Allan, Christopher H.;Fleckman, Philip;Clark, John M.
哺乳动物指尖的再生已有详细描述；然而，相关的细胞或分子事件尚未在人类中进行研究。我们描述了对指尖截肢反应的体外人类胎儿模型，并报告了转录抑制子 Msx1 在发育和再生的人类指尖中的表达。来自估计孕龄 (EGA) 53 至 117 天样本的人类胎儿手指在补充氧气的限定无血清培养基中培养 4 天至 4 周。对配对对照和尖端截肢的手指进行组织学和免疫组织化学分析。在 EGA 的 80 天里，再生组织覆盖了远端指骨的切口端。 Msx1 表达在至少 70 天 EGA 的对照手指的指甲区域下方以及截肢后 4 和 7 天的 57 天手指的再生尖端处检测到。我们的研究结果表明，人类胎儿的手指在截肢后会在体外重新生长组织。该过程在空间上与 Msx1 表达相关。截肢后 4 天，57 天手指再生尖端的 Msx1 表达似乎有所增加。
Regeneration of mammalian digit tips is well described; however, associated cellular or molecular events have not been studied in humans. We describe an in vitro human fetal model of response to digit tip amputation, and report expression of the transcription repressor Msx1 in the developing and regrowing human digit tip. Human fetal digits from specimens ranging from 53 to 117 days' estimated gestational age (EGA) were cultured in a defined serum-free medium with supplemented oxygen for time periods from 4 days to 4 weeks. Histology and immunohistochemistry were performed on paired control and tip-amputated digits. Regrowing tissue covered the cut end of the distal phalanx in digits up to 80 days' EGA. Msx1 expression was detected beneath the nail field in control digits to at least 70 days' EGA and at the regrowing tip of 57-day digits at 4 and 7 days post-amputation. Our results show that human fetal digits regrow tissue in vitro in response to tip amputation. This process appears spatially associated with Msx1 expression. Msx1 expression appears increased at the regrowing tip of 57-day digits by 4 days after amputation.