Production of interferon gamma and interleukin-2 by peripheral blood lymphocytes of patients with myasthenia gravis and other autoimmune diseases.
Production of interferon gamma and interleukin-2 by peripheral blood lymphocytes of patients with myasthenia gravis and other autoimmune diseases.
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重症肌无力和其他自身免疫性疾病患者外周血淋巴细胞产生干扰素 γ 和白细胞介素 2。
DOI：
10.1111/j.1749-6632.1993.tb22905.x
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发表时间：
1993
影响因子：
5.2
通讯作者：
Grob,D
中科院分区：
文献类型：
--
作者：
Mokhtarian,F;Shirazian,D;Grob,D
研究了在AChR缺失和IL-2存在的情况下，从重症肌无力（MG）患者、其他自身免疫性疾病（OAD）患者和正常人外周血中建立的t细胞系产生干扰素γ (IFN-g)和白细胞介素2 （IL-2）的情况。仅培养一周后，MG患者的T细胞产生的IFN-g和IL-2低于OAD和正常对照的T细胞。然而，随后，MG和OAD患者的T细胞的IFN-g和IL-2的产生增加，且明显高于正常对照。免疫调节缺陷可能在MG和OAD患者的T细胞中起作用，导致激活和淋巴因子产生的爆发，这可能在这些疾病的发病机制中发挥作用。重症肌无力被认为是一种主要由乙酰胆碱受体（AChR）抗体介导的自身免疫性疾病。“然而，疾病的严重程度与血清抗体水平并不相关。”“包括我们在内的许多研究发现，MG患者外周血中的T细胞通过淋巴细胞增殖，对AChR或AChR合成肽的反应比正常的T细胞更大。然而，这些细胞系都是通过带抗原的T细胞的体外培养获得的，因此，这些T细胞的特定功能及其参与调节自身抗体产生的作用尚未完全阐明。”抗体滴度与疾病严重程度之间缺乏相关性表明，其他因素，如细胞因子的产生，可能在决定所产生疾病的严重程度方面发挥重要作用。
The production of interferon gamma (IFN-g) and interleukin-2 (IL-2) by T-cell lines established from the peripheral blood of patients with myasthenia gravis (MG), other autoimmune diseases (OAD), and normal controls, in the absence of AChR and in the presence of IL-2, was investigated. Production, after only one week in culture, of IFN-g and IL-2 by T cells of patients with MG was lower than by T cells of OAD and normal controls. Later, however, the production of IFN-g and IL-2 increased and was significantly higher by T cells of MG and OAD patients than by normal controls. An immunoregulatory defect may be operating in the T cells of MG and OAD patients that results in an outbprst of activation and lymphokine production, which may play a role in the pathogenesis of these diseases.Myasthenia gravis is thought to be an autoimmune disease primarily mediated by antibodies to the acetylcholine receptor (AChR).'The severity of disease, however, does not correlate well with serum antibody levels.'Many studies, including ours, have found that T cells from the peripheral blood of MG patients respond, by lymphoproliferation, to AChR or to AChR synthetic peptides, to a greater extent than T cells from normal These lines, however, were all obtained by in uitro culture of T cells with antigens, Thus, the specific function of these T cells, and their involvement in the regulation of autoantibody production, has not been fully elucidated. The lack of correlation between antibody titer and disease severity suggests that other factors, such as cytokine production, may play an important role in determining the severity of the disease produced.