Veillonella seminalis sp nova, a novel anaerobic Gram-stain-negative coccus from human clinical samples, and emended description of the genus Veillonella
Veillonella seminalis sp nova, a novel anaerobic Gram-stain-negative coccus from human clinical samples, and emended description of the genus Veillonella
复制标题
DOI：
10.1099/ijs.0.064451-0
复制
发表时间：
2014-10-01
影响因子：
2.8
通讯作者：
Marchandin, Helene
中科院分区：
文献类型：
--
作者：
Aujoulat, Fabien;Bouvet, Philippe;Marchandin, Helene
从人体中回收了10株未知的革兰氏阴性厌氧菌。临床样本，主要来自精液。根据其表型特征，包括形态、主要代谢终产物、产气、硝酸盐还原和琥珀酸脱碳，将这些菌株鉴定为韦荣氏球菌属成员。基于16 S rRNA、dnaK和rpoB基因以及新提出的gltA基因进行了多位点序列分析和相应的同源性分析。这些菌株具有高度的遗传序列相似性，与Veillonella ratti关系最密切。16 SrRNA基因序列分析表明，该菌株与V. ratti不能区分，而gltA、rpoB和dnaK基因序列与V. ratti模式株的相似性分别为85.1%、93.5%和90.2%。系统发育分析表明，分离物形成了一个强大的分支，在Veillonella属的Veillonella rati-Veillonella criceti Veillonella magna亚组。如对蟋蟀弧菌所观察到的，分离物能够发酵果糖。与韦荣氏球菌属的其他成员相比，这10种菌株不能代谢乳酸。细胞脂肪酸组成与韦荣氏球菌属的其他物种是一致的。根据这些数据，10个分离株被认为属于韦荣氏球菌属中的一个新种，建议将其命名为Veillonella vestralis sp. nov.。模式株为ADV 4313.2（T）（=CIP 107810（T）=LMG 28162（T））。作为人类微生物组计划的一部分，进行全基因组测序的韦荣氏球菌菌株ACS-216-V-Col 6 b是V. vestialis sp. nov.的另一个代表。
Ten isolates of unknown, Gram-stain-negative, anaerobic cocci were recovered from human. clinical samples, mainly from semen. On the basis of their phenotypic features, including morphology, main metabolic end products, gas production, nitrate reduction and decarbondation of succinate, the strains were identified as members of the genus Veillonella. Multi-locus sequence analysis and corresponding phylogenies were based on 16S rRNA, dnaK and rpoB genes, and on the newly proposed gltA gene. The strains shared high levels of genetic sequence similarity and were related most closely to Veillonella ratti. The strains could not be differentiated from V. ratti on the basis of 16S rRNA gene sequence analysis while gltA, rpoB and dnaK gene sequences showed 85.1, 93.5 and 90.2% similarity with those of the type strain of V. ratti, respectively. Phylogenetic analyses revealed that the isolates formed a robust clade in the V. ratti-Veillonella criceti Veillonella magna subgroup of the genus Veillonella. As observed for V. criceti, the isolates were able to ferment fructose. In contrast to other members of the genus Veillonella, the 10 strains were not able to metabolize lactate. Cellular fatty acid composition was consistent with that of other species of the genus Veillonella. From these data, the 10 isolates are considered to belong to a novel species in the genus Veillonella, for which the name Veillonella seminalis sp. nov. is proposed. The type strain is ADV 4313.2(T) (=CIP 107810(T)=LMG 28162(T)). Veillonella strain ACS-216-V-Col6b subjected to whole genome sequencing as part as the Human Microbiome Project is another representative of V. seminalis sp. nov. An emended description of the genus Veillonella is also proposed.