Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells
Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells
复制标题
人乳头瘤病毒 16 E6 与食管癌细胞的核基质相关。
DOI：
10.3748/wjg.v7.i6.788
复制
发表时间：
2001-12-01
影响因子：
4.3
通讯作者：
Chew, EC
中科院分区：
文献类型：
--
作者：
Chen, HB;Chen, L;Chew, EC
目的：探讨人乳头瘤病毒(HPV)感染在食管癌发病机制中的作用，以及HPV-16E6与癌细胞核基质的关系。方法：采用聚合酶链式反应(PCR)扩增病毒DNA相关核基质，对EC/CUHK1和EC/CUHK2两株食管癌细胞株进行HPV-16E6亚基因片段检测。结果：在EC/CUHK2细胞中发现HPV-16E6亚基因片段存在于核基质相关DNA中，E6癌蛋白定位于与核基质密切相关的细胞核中。结论：HPV-16E6与核基质的相互作用可能参与了HPV-16E6的致癌作用。
AIM: To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells.METHODS: Two esophageal carcinoma cell lines, EC/ CUHK1 and EC/CUHK2, were tested for HPV-16 E6 subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualize the expression of E6 subgene in the cells.RESULTS: The HPV-16 E6 subgenetic fragment was found to be present in nuclear matrix-associated DNA, E6 oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. it was not detected, however, in EC/ CUHK1 cells.CONCLUSION: The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.