Enhanced uptake of glycerol by butyrate treatment in HCT-15 human colon cancer cell line
Enhanced uptake of glycerol by butyrate treatment in HCT-15 human colon cancer cell line
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DOI：
10.2133/dmpk.22.195
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发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Yuasa, Hiroaki
中科院分区：
文献类型：
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作者：
Fujimoto, Nami;Inoue, Katsuhisa;Yuasa, Hiroaki
HCT-15 人结肠癌细胞系具有 Na+ 依赖性载体介导的甘油转运系统。类似的转运系统也被认为存在于小肠中，并且因其在甘油和可能的一些结构相关化合物的吸收中的作用而令人感兴趣。为了帮助阐明此类甘油转运系统的功能特征，我们研究了丁酸盐（一种已知促进细胞分化的试剂）对 HCT-15 细胞甘油摄取的影响。发现用丁酸盐 (2 mM) 预处理 24 小时的 HCT-15 细胞中甘油 (0.4 mM) 的摄取量比未处理的细胞高约 5 倍。丁酸盐处理的吸收增加是由于最大转运速率的增加。在丁酸盐处理过程中，当向培养基中添加基因转录抑制剂放线菌素 D 和蛋白质合成抑制剂放线菌酮时，丁酸盐的作用几乎完全被抑制。这些结果支持这样的建议，即特定的载体蛋白参与 HCT-15 细胞的甘油摄取，并且该载体蛋白是由丁酸盐诱导的细胞分化诱导的蛋白之一。
The HCT-15 human colon cancer cell line has a Na+- dependent carrier-mediated transport system for the uptake of glycerol. A similar transport system has been suggested to be present also in the small intestine and is of interest with regard to its role in the absorption of glycerol and possibly some structurally related compounds. To help clarifying functional characteristics of such glycerol transport systems, we examined the effect of butyrate, an agent known to facilitate the differentiation of cells, on glycerol uptake in HCT-15 cells. The uptake of glycerol (0.4 mM) was found to be about 5-fold greater in HCT-15 cells pretreated with butyrate (2 mM) for 24 h than in those untreated. The increase in the uptake by the butyrate treatment was due to an increase in the maximum transport rate. The effect of butyrate was almost completely suppressed when actinomycin D, an inhibitor of gene transcription, and cycloheximide, an inhibitor of protein synthesis, were added to the medium during the butyrate treatment. These results support the suggestion that a specific carrier protein is involved in glycerol uptake by HCT-15 cells and the carrier protein is one of those inducible by butyrate-induced cell differentiation.