Current Status of Point-of-Care Testing for Human Immunodeficiency Virus Drug Resistance
Current Status of Point-of-Care Testing for Human Immunodeficiency Virus Drug Resistance
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DOI：
10.1093/infdis/jix413
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发表时间：
2017-11-15
影响因子：
6.4
通讯作者：
Frenkel, Lisa M.
中科院分区：
文献类型：
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作者：
Duarte, Horacio A.;Panpradist, Nuttada;Frenkel, Lisa M.
由于实施了即时检验，医疗服务有所进步，这种检验通常在设备简陋的实验室或在家中在几分钟到几小时内就能完成。技术进步催生了包含基于核酸的检测方法（包括聚合酶链反应、等温扩增、连接和杂交反应）的即时检验试剂盒。由于少数单核苷酸多态性与具有临床意义的人类免疫缺陷病毒（HIV）耐药性相关，因此已经开发出检测这些突变的检测方法。这些检测方法的早期版本已用于研究。这篇综述总结了每种检测方法的基本原理，并讨论了将它们纳入HIV感染管理的策略需求。
Healthcare delivery has advanced due to the implementation of point-of-care testing, which is often performed within minutes to hours in minimally equipped laboratories or at home. Technologic advances are leading to point-of-care kits that incorporate nucleic acid-based assays, including polymerase chain reaction, isothermal amplification, ligation, and hybridization reactions. As a limited number of single-nucleotide polymorphisms are associated with clinically significant human immunodeficiency virus (HIV) drug resistance, assays to detect these mutations have been developed. Early versions of these assays have been used in research. This review summarizes the principles underlying each assay and discusses strategic needs for their incorporation into the management of HIV infection.