Detection of Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
Detection of Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, Borrelia miyamotoi, and Powassan Virus in Ticks by a Multiplex Real-Time Reverse Transcription-PCR Assay
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DOI：
10.1128/msphere.00151-17
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发表时间：
2017-03-01
期刊：
影响因子：
4.8
通讯作者：
Lipkin, W. Ian
中科院分区：
文献类型：
--
作者：
Tokarz, Rafal;Tagliafierro, Teresa;Lipkin, W. Ian
肩胛骨硬蜱与嗜吞噬细胞无形体、伯氏疏螺旋体、宫本疏螺旋体、微小巴贝斯虫和波瓦桑病毒的传播有关。我们描述了建立和实施第一个多重实时PCR测定，能够同时检测和区分所有五种病原体在一个单一的反应。应用该方法对纽约和康涅狄格的蜱虫进行分析，发现纽约州萨福克县的蜱虫中有高流行率的微蜱。这些发现与该地区管理蜱传疾病患者护理的临床医生报告的巴贝斯虫病发病率较高相一致。了解病原体流行情况是确定蜱传疾病的人类风险的重要因素，有助于指导诊断和治疗。我们的检测方法的实施解决了蜱传疾病监测的关键需求，通过对肩胛骨蜱病原体流行率进行全面评估。我们在纽约州萨福克县发现蜱虫感染巴贝虫的频率很高，这表明该媒介可能是该地区非莱姆病蜱传播疾病的常见原因。
Ixodes scapularis ticks are implicated in transmission of Anaplasma phagocytophilum, Borrelia burgdorferi, Borrelia miyamotoi, Babesia microti, and Powassan virus. We describe the establishment and implementation of the first multiplex real-time PCR assay with the capability to simultaneously detect and differentiate all five pathogens in a single reaction. The application of this assay for analysis of ticks at sites in New York and Connecticut revealed a high prevalence of B. microti in ticks from Suffolk County, NY. These findings are consistent with reports of a higher incidence of babesiosis from clinicians managing the care of patients with tick-borne diseases in this region.IMPORTANCE The understanding of pathogen prevalence is an important factor in the determination of human risks for tick-borne diseases and can help guide diagnosis and treatment. The implementation of our assay addresses a critical need in surveillance of tick-borne diseases, through generation of a comprehensive assessment of pathogen prevalence in I. scapularis. Our finding of a high frequency of ticks infected with Babesia microti in Suffolk County, NY, implicates this agent as a probable frequent cause of non-Lyme tick-borne disease in this area.