Spectroscopically determined collagen Pyr/deH-DHLNL cross-link ratio and crystallinity indices differ markedly in recombinant congenic mice with divergent calculated bone tissue strength
Spectroscopically determined collagen Pyr/deH-DHLNL cross-link ratio and crystallinity indices differ markedly in recombinant congenic mice with divergent calculated bone tissue strength
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DOI：
10.1080/03008200390223918
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发表时间：
2003-05-01
影响因子：
2.9
通讯作者：
Paschalis, EP
中科院分区：
文献类型：
--
作者：
Blank, RD;Baldini, TH;Paschalis, EP
全骨强度可分为结构和材料成分。在HcB/Dem重组同源系列的6个月大雌性肱骨的三点弯曲试验中，菌株HcB/8和HcB/23在计算的破坏应力方面存在显著差异，但灰分百分比没有差异。傅立叶变换红外光谱成像用于确定吡啶啉（pyr;不可还原的）与脱氢二羟基正亮氨酸（de-DHLNL;可还原的）胶原交联（XLR）的比率、矿物结晶度或空间有序性的差异是否可以解释菌株不同的生物力学性能。与HcB/23相比，HcB/8具有显著更高的XLR和显著更高的结晶度。XLR和结晶度是高度和相似的相关性在这两个菌株。各菌株的一维空间相关函数差异不显著，表明它们之间的短程有序性没有差异。XLR和结晶度之间的强相关性反映了骨的蛋白质和矿物元素的相互依赖性。这些数据说明了材料特性以及矿物质含量对骨组织强度的重要性。
Whole bone strength can be partitioned into structural and material components. In three-point bending tests of 6-month-old female humeri from the HcB/Dem recombinant congenic series, strains HcB/8 and HcB/23 differed markedly in calculated failure stress but not ash percentage. Fourier transform infrared spectroscopic imaging was used to determine whether differences in the ratio of pyridinoline (pyr; nonreducible) to dehydrodihydroxynorleucine (de-DHLNL; reducible) collagen cross-links (XLR), mineral crystallinity, or spatial ordering could account for the strains' differing biomechanical performance. HcB/8 had significantly higher XLR and significantly higher crystallinity than HcB/23. XLR and crystallinity were highly and similarly correlated in both strains. There were no significant differences between the strains' one-dimensional spatial correlation functions, suggesting no difference in short-range order between them. The strong correlation between XLR and crystallinity reflects the interdependence of the protein and mineral elements of bone. The data illustrate the importance of material properties in addition to mineral quantity to bone tissue strength.