MOUNTING OF CRYSTALS FOR MACROMOLECULAR CRYSTALLOGRAPHY IN A FREESTANDING THIN-FILM
MOUNTING OF CRYSTALS FOR MACROMOLECULAR CRYSTALLOGRAPHY IN A FREESTANDING THIN-FILM
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DOI：
10.1107/s0021889890005568
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发表时间：
1990-10-01
影响因子：
6.1
通讯作者：
TENG, TY
中科院分区：
文献类型：
--
作者：
TENG, TY
描述了一种在大分子晶体学研究中安装单晶的方法，其中晶体悬浮在薄膜中。该薄膜由结晶缓冲液和亲水性粘性材料的混合物形成，通过表面张力限制在细线环内。与传统的晶体安装方法相比，该方法大大简化并加快了安装过程，非常适合晶体的冲击冷冻和光学监测，使脆弱的晶体变形更少，并在 X 射线衍射图案中提供更低且更均匀的背景。
A method for mounting single crystals in macromolecular crystallographic studies is described in which the crystal is suspended in a thin film. The film is formed from a mixture of the crystallization buffer and a hydrophilic viscous material, confined within a thin-wire loop by surface tension. Compared with conventional crystal mounting methods, this method greatly simplifies and speeds the mounting procedure, is well suited to shock freezing and to optical monitoring of the crystals, deforms fragile crystals less and gives a lower and more uniform background in the X-ray diffraction patterns.