Identification of the Interaction between P-Glycoprotein and Anxa2 in Multidrug-resistant Human Breast Cancer Cells.
Identification of the Interaction between P-Glycoprotein and Anxa2 in Multidrug-resistant Human Breast Cancer Cells.
复制标题
多重耐药人乳腺癌细胞中 P-糖蛋白与 Anxa2 相互作用的鉴定
DOI：
10.3969/j.issn.2095-3941.2012.02.003
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发表时间：
2012-06
影响因子：
5.5
通讯作者：
Niu RF
中科院分区：
文献类型：
--
作者：
Zhang HC;Zhang F;Wu B;Han JH;Ji W;Zhou Y;Niu RF
目的探讨Anxa2与P-糖蛋白(P-gp)在多药耐药(MDR)人乳腺癌细胞株MCF-7/ADR迁移侵袭中的相互作用。方法将一对针对P-gp的短发夹状RNA(ShRNA)导入MCF-7/ADR细胞，筛选细胞株。Western印迹法检测P-gp的表达。Transwell小室观察细胞的迁移能力和侵袭能力。免疫沉淀和免疫荧光共聚焦显微镜观察P-gp与Anxa2的相互作用。结果MDR乳腺癌细胞P-gp表达明显下调，迁移和侵袭能力有显著下降趋势(P<；0.05)。Anxa2与P-gp之间存在密切的相互作用。结论MCF-7/ADR是一株多药耐药的人乳腺癌细胞系，具有较强的侵袭和迁移能力。P-gp基因的敲除显著削弱了肿瘤细胞的迁移和侵袭能力。Anxa2与P-PG的相互作用可能在多药耐药人乳腺癌细胞侵袭力增强中起重要作用。
Objective To explore the interaction of Anxa2 with P-Glycoprotein (P-gp) in the migration and invasion of the multidrug-resistant (MDR) human breast cancer cell line MCF-7/ADR. Methods A pair of short hairpin RNA (shRNA) targeting P-gp was transfected into MCF-7/ADR cells, and monoclonal cell strains were screened. The expression of P-gp was detected by Western blot. Transwell chambers were used to observe the cell migration capacity and invasion ability. The interaction between P-gp and Anxa2 was examined by immunoprecipitation and immunofluorescence confocal microscopy analyses. Results P-gp expression was significantly knocked down, and there were notable decreasing trends in the migration and invasion capability of MDR breast cancer cells (P<0.05). There was a close interaction between Anxa2 and P-gp. Conclusions MCF-7/ADR is an MDR human breast cancer cell line with high migration and invasion abilities. The knockdown of P-gp notably impaired the migration and invasion abilities of the tumor cells. The interaction of Anxa2 with P-pg may play an important role in the enhanced invasiveness of MDR human breast cancer cells.
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影响因子：
11.5
作者：
Reimers, N;Zafrakas, K;Pantel, K
通讯作者：
Pantel, K
影响因子：
5.6
作者：
THOMPSON, EW;PAIK, SM;DICKSON, RB
通讯作者：
DICKSON, RB
影响因子：
11.2
作者：
Miletti-González, KE;Chen, SL;Rodríguez-Rodríguez, L
通讯作者：
Rodríguez-Rodríguez, L
Anxa2 在增强多药耐药人类乳腺癌细胞的侵袭性方面发挥关键作用
影响因子：
4.4
作者：
Zhang, Fei;Zhang, Lin;Niu, Ruifang
通讯作者：
Niu, Ruifang
影响因子：
4.8
作者：
Bajorath, J;Greenfield, B;Aruffo, A
通讯作者：
Aruffo, A