Syntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate
Syntrophic co-culture of a methanotroph and heterotroph for the efficient conversion of methane to mevalonate
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DOI：
10.1016/j.ymben.2021.07.008
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发表时间：
2021-07-24
影响因子：
8.4
通讯作者：
Lee, Seung-Goo
中科院分区：
文献类型：
--
作者：
Lee, Hyewon;Baek, Ji In;Lee, Seung-Goo
随着甲烷的生物转化在生物工业和环境应用中变得越来越重要，甲烷氧化菌因其在环境条件下转化甲烷的能力而受到广泛关注。这包括广泛报道甲烷转化为生物化学品的甲烷氧化菌工程。为了进一步提高甲烷的可用性，我们展示了一种高度灵活和有效的模块化方法，该方法基于甲烷氧化菌和异养菌的合成财团，模仿天然甲烷生态系统，从甲烷中生产甲羟戊酸（MVA）。在甲烷转化模块中，我们使用荚膜甲基球菌Bath作为高效甲烷生物催化剂，并优化了生产大量有机酸的培养条件。在MVA合成模块中，我们使用具有高有机酸耐受性和利用能力的进化菌株Escherichia coli SBA 01将有机酸转化为MVA。利用重组E. coli SBA 01，在48 h内，在不添加除甲烷以外的任何营养物质的情况下，成功地产生了61 mg/L（0.4 mM）的MVA。我们的平台在细胞生长和MVA生产方面表现出高稳定性和可重复性。我们相信，这种多功能系统可以很容易地扩展到许多其他增值过程，并具有各种潜在的应用。
As the bioconversion of methane becomes increasingly important for bio-industrial and environmental applications, methanotrophs have received much attention for their ability to convert methane under ambient conditions. This includes the extensive reporting of methanotroph engineering for the conversion of methane to biochemicals. To further increase methane usability, we demonstrated a highly flexible and efficient modular approach based on a synthetic consortium of methanotrophs and heterotrophs mimicking the natural methane ecosystem to produce mevalonate (MVA) from methane. In the methane-conversion module, we used Methylococcus capsulatus Bath as a highly efficient methane biocatalyst and optimized the culture conditions for the production of high amounts of organic acids. In the MVA-synthesis module, we used Escherichia coli SBA01, an evolved strain with high organic acid tolerance and utilization ability, to convert organic acids to MVA. Using recombinant E. coli SBA01 possessing genes for the MVA pathway, 61 mg/L (0.4 mM) of MVA was successfully produced in 48 h without any addition of nutrients except methane. Our platform exhibited high stability and reproducibility with regard to cell growth and MVA production. We believe that this versatile system can be easily extended to many other value-added processes and has a variety of potential applications.