Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images
Validation of luminescent source reconstruction using single-view spectrally resolved bioluminescence images
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DOI：
10.1364/ao.46.002540
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发表时间：
2007-05-01
期刊：
影响因子：
1.9
通讯作者：
Jansen, E. Duco
中科院分区：
文献类型：
--
作者：
Virostko, John;Powers, Alvin C.;Jansen, E. Duco
我们的特点的能力和局限性的生活图像软件三维分析包（Xenogen，阿拉米达，加州）在重建校准光源。浅于光传播的平均自由程的源遭受重建不准确。对于深于平均自由程的源，对于均匀组织，深度和强度重建的平均误差分别小于4%和12%。植入光学异质性小鼠腹部的发光珠的重建被证明不太准确。随着震源深度的增加，区分多个震源的能力下降。许多因素影响光源重建的准确性。(c)2007年，美国光学学会。
We characterize the capabilities and limitations of the Living Image Software 3D Analysis package (Xenogen, Alameda, California) in the reconstruction of calibrated light sources. Sources shallower than the mean free path of light propagation suffered reconstruction inaccuracy. For sources deeper than the mean free path, the average error in depth and intensity reconstruction was less than 4% and 12%, respectively, for homogeneous tissue. The reconstruction of luminescent beads implanted within an optically heterogeneous mouse abdomen proved less accurate. The ability to distinguish multiple sources decreased with increasing source depth. A number of factors influence the accuracy of light source reconstruction. (c) 2007 Optical Society of America.