Bile Acids Quantification by Liquid Chromatography-Tandem Mass Spectrometry: Method Validation, Reference Range, and Interference Study
Bile Acids Quantification by Liquid Chromatography-Tandem Mass Spectrometry: Method Validation, Reference Range, and Interference Study
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DOI：
10.3390/diagnostics10070462
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发表时间：
2020-07-01
期刊：
影响因子：
3.6
通讯作者：
Lippi, Giuseppe
中科院分区：
文献类型：
--
作者：
Danese, Elisa;Negrini, Davide;Lippi, Giuseppe
胆汁酸（BA）在胆固醇代谢中发挥着关键作用。它们的血液浓度也被提议作为肝胆、肠道和心血管疾病的新预后和诊断指标。液相色谱串联质谱 (LC-MS/MS) 目前代表了生物样品中 BA 谱分析的黄金标准。我们在此报告了 LC-MS/MS 技术的开发和验证，该技术可同时定量血清样品中的 15 种 BA 物种。我们还建立了成年健康受试者（n=130）的参考范围，并对体外和体内干扰进行了初步评估。该方法显示出良好的线性，所有测试分析物在 5 ng/mL（定量下限，LLOQ）和 5000 ng/mL 范围内具有高回归系数 (>0.99)。准确度在 85-115% 之间。测定内和测定间的不精密度均
Bile acids (BA) play a pivotal role in cholesterol metabolism. Their blood concentration has also been proposed as new prognostic and diagnostic indicator of hepatobiliary, intestinal, and cardiovascular disease. Liquid chromatography tandem mass spectrometry (LC-MS/MS) currently represents the gold standard for analysis of BA profile in biological samples. We report here development and validation of a LC-MS/MS technique for simultaneously quantifying 15 BA species in serum samples. We also established a reference range for adult healthy subjects (n= 130) and performed a preliminary evaluation of in vitro and in vivo interference. The method displayed good linearity, with high regression coefficients (>0.99) over a range of 5 ng/mL (lower limit of quantification, LLOQ) and 5000 ng/mL for all analytes tested. The accuracies were between 85-115%. Both intra- and inter-assay imprecision was