Two-photon laser scanning microscopy with electrowetting-based prism scanning
Two-photon laser scanning microscopy with electrowetting-based prism scanning
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DOI：
10.1364/boe.8.005412
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发表时间：
2017-12-01
影响因子：
3.4
通讯作者：
Bright, Victor M.
中科院分区：
文献类型：
--
作者：
Supekar, Omkar D.;Ozbay, Baris N.;Bright, Victor M.
激光扫描仪是高分辨率生物医学成像系统的一个组成部分，如共聚焦或2光子激发（2PE）显微镜。在这项工作中，我们展示了电介质上的电润湿（EWOD）棱镜作为集成在传统2PE显微镜中的横向激光扫描元件的实用性。据我们所知，这是EWOD棱镜的首次演示。EWOD器件提供了一种传输、低功耗和紧凑的替代传统自适应光学器件，因此这项技术具有巨大的潜力。我们展示了用EWOD棱镜扫描在视场为130 × 130 μ m(2)的2PE显微镜下对培养的小鼠海马神经元的成像。此外，我们用EWOD棱镜模拟了光学系统，以评估高斯光束通过EWOD棱镜传播对成像质量的影响。根据仿真结果，选择光束尺寸为0.91 mm全宽半max进行成像实验，得到成像系统的数值孔径为0.17。(C) 2017年美国光学学会根据OSA开放获取出版协议的条款
Laser scanners are an integral part of high resolution biomedical imaging systems such as confocal or 2-photon excitation (2PE) microscopes. In this work, we demonstrate the utility of electrowetting on dielectric (EWOD) prisms as a lateral laser-scanning element integrated in a conventional 2PE microscope. To the best of our knowledge, this is the first such demonstration for EWOD prisms. EWOD devices provide a transmissive, low power consuming, and compact alternative to conventional adaptive optics, and hence this technology has tremendous potential. We demonstrate 2PE microscope imaging of cultured mouse hippocampal neurons with a FOV of 130 x 130 mu m(2 )using EWOD prism scanning. In addition, we show simulations of the optical system with the EWOD prism, to evaluate the effect of propagating a Gaussian beam through the EWOD prism on the imaging quality. Based on the simulation results a beam size of 0.91 mm full width half max was chosen to conduct the imaging experiments, resulting in a numerical aperture of 0.17 of the imaging system. (C) 2017 Optical Society of America under the terms of the of the OSA Open Access Publishing Agreement