Metabolic Changes of Maternal Uterine Fluid, Uterus, and Plasma during the Peri-implantation Period of Early Pregnancy in Mice
Metabolic Changes of Maternal Uterine Fluid, Uterus, and Plasma during the Peri-implantation Period of Early Pregnancy in Mice
复制标题
小鼠妊娠早期围着床期母体子宫液、子宫和血浆的代谢变化。
DOI：
10.1007/s43032-019-00040-5
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发表时间：
2020
影响因子：
2.9
通讯作者：
Ting-Li Han
中科院分区：
文献类型：
--
作者：
Yang Yang;Longqiong Wang;Chang Chen;Hongbo Qi;Philip N.Baker;Xueqing Liu;Hua Zhang;Ting-Li Han
胚胎着床是一个复杂的过程，涉及着床能力强的胚泡和接受子宫之间的生化和生理相互作用。然而，围植入期子宫液、子宫和血浆的确切生化变化仍不清楚。本研究以小鼠为动物模型，研究早期妊娠着床期的生化和代谢变化。用气相色谱-质谱仪分析植入前和植入时子宫、子宫液和母体血浆的代谢物分布。多变量分析，方差分析和Tukey‘s HSD检验，被用来检测代谢物和代谢途径的显著变化。根据已鉴定的代谢物和KEGG代谢框架，在Silico中重建代谢网络。在着床前第1天和第4天之间，观察到子宫液中发生了戏剧性的代谢变化，这对胚泡发育和抵御恶劣子宫环境的保护可能是重要的。棕榈油酸、延胡索酸和戊二酸在子宫中的水平在第4天发生变化，这表明它们可能与子宫内膜容受性有关。在着床早期，子宫和母体的细胞代谢都发生了深刻的变化，包括支链氨基酸和一碳代谢中间产物的上调，乙醛酸和二羧酸代谢的上调，有氧呼吸的下调，所有这些都参与了母胎界面的调节，营养物质的替代利用，植入的能量保存，以及后来的胎盘形成和胎儿发育，以确保胚胎着床成功。
Embryo implantation is a complex process which involves biochemical and physiological interactions between an implantation-competent blastocyst and a receptive uterus. However, the exact biochemical changes of uterine fluid, uterus, and plasma during peri-implantation remain unclear. This study aims to characterize the biochemical and metabolic changes that occur during the peri-implantation period of early pregnancy, using mice as an animal model. Gas chromatography-mass spectrometry was used to analyze the metabolite profiles of the uterus, uterine fluid, and maternal plasma at pre-implantation and implantation. The multivariate analyses, ANOVA and Tukey’s HSD test, were applied to detect significant changes in metabolites and metabolic pathways. The metabolic networks were reconstructed in silico based on the identified metabolites and KEGG metabolic framework. Between pre-implantation day 1 and day 4, dramatic metabolic changes were observed in the uterine fluid that could be important for blastocyst development and protection against the harsh uterine environment. Palmitoleic acid, fumaric acid, and glutaric acid changed levels at day 4 in the uterus, suggesting that they may be associated with endometrial receptivity. Both the uterus and maternal plasma showed profound changes in cellular metabolism at the early implantation period, including upregulation of branched-chain amino acids and intermediates of one-carbon metabolism, an upregulation of glyoxylate and dicarboxylate metabolism, and downregulation of aerobic respiration; all of which could be involved in the regulation of the maternal-fetal interface, alternative nutrient utilization, and energy preservation for implantation as well as later placentation and fetal development to ensure successful embryo implantation.