Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells
Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells
复制标题
DOI：
10.1634/stemcells.2004-0021
复制
发表时间：
2005-03-01
期刊：
影响因子：
5.2
通讯作者：
Ogle, RC
中科院分区：
文献类型：
--
作者：
Katz, AJ;Tholpady, A;Ogle, RC
成人皮下脂肪组织含有具有有趣的多谱系发育可塑性的细胞，很像骨髓间充质干细胞。文献中假定的来自脂肪的干细胞或祖细胞被赋予了许多不同的名称，反映了关于其表型特征的早期和不断发展的共识。此处报道的研究使用微阵列评估了从三个独立供体分离的未分化、早期传代人脂肪源性贴壁基质 (hADAS) 细胞中与血管生成和细胞外基质相关的 170 多个基因。 hADAS 群体一致转录了 66% 的筛选基因，并且 83% 由三个群体中的至少两个转录。转录程度最高的基因与功能分组相关，例如细胞粘附、基质蛋白、生长因子和受体以及蛋白酶。这项工作证明的 hADAS 细胞转录组揭示了与已发表的骨髓间充质干细胞 (MSC) 概况的许多相似之处。此外，尽管命名不一致且缺乏细胞分离和培养的标准化方案，但对超过 24 个 hADAS 细胞表面蛋白（n = 7 个供体）的流式分析证实并扩展了现有文献，并揭示了强烈的组间相关性。最后，基于流式分析和逆转录聚合酶链式反应研究，我们的结果表明，hADAS 细胞不表达其他干细胞群中作为“干性”标记的几种蛋白质，包括端粒酶、CD133 和膜转运蛋白 ABCG2。
Adult human subcutaneous adipose tissue contains cells with intriguing multilineage developmental plasticity, much like marrow-derived mesenchymal stem cells. Putative stem or progenitor cells from fat have been given many different names in the literature, reflecting an early and evolving consensus regarding their phenotypic characterization. The study reported here used microarrays to evaluate over 170 genes relating to angiogenesis and extracellular matrix in undifferentiated, early-passage human adipose-derived adherent stromal (hADAS) cells isolated from three separate donors. The hADAS populations unanimously transcribed 66% of the screened genes, and 83% were transcribed by at least two of the three populations. The most highly transcribed genes relate to functional groupings such as cell adhesion, matrix proteins, growth factors and receptors, and proteases. The transcriptome of hADAS cells demonstrated by this work reveals many similarities to published profiles of bone marrow mesenchymal stem cells (MSCs). In addition, flow analysis of over 24 hADAS cell surface proteins (n = 7 donors) both confirms and expands on the existing literature and reveals strong intergroup correlation, despite an inconsistent nomenclature and the lack of standardized protocols for cell isolation and culture. Finally, based on flow analysis and reverse transcription polymerase chain reaction studies, our results suggest that hADAS cells do not express several proteins that are implicated as markers of "stemness" in other stem cell populations, including telomerase, CD133, and the membrane transporter ABCG2.