Commensal bacteria influence innate status within gingival tissues: A pilot study
Commensal bacteria influence innate status within gingival tissues: A pilot study
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DOI：
10.1902/jop.2004.75.11.1486
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发表时间：
2004-11-01
影响因子：
4.3
通讯作者：
Darveau, RP
中科院分区：
文献类型：
--
作者：
Dixon, DR;Reife, RA;Darveau, RP
背景：本研究的目的是通过检测BALBc/ByJ和SCID C.B17小鼠无菌组和常规饲养组中先天宿主防御介质的表达，确定共生菌对牙龈组织先天防御状态的贡献。方法：采用半定量反转录聚合酶链反应（RT-PCR）测定各牙龈组织组（N = 5）内e-选择素、p -选择素、白细胞介素-(IL)-8同源物、肿瘤坏死因子- α、IL-1 β、细胞间粘附分子-(ICAM)-1、ICAM-2、血管粘附分子-(VCAM)-1的组成水平。采用酶联免疫吸附法（ELISA）测定IL-1蛋白含量。结果：常规饲养小鼠的牙龈样本显示，在所有检测的介质中，只有il -1 β mRNA的表达显著降低(P
Background: The objective of this study was to determine the contribution of commensal bacteria to the innate defense status of gingival tissue by examining the expression of innate host defense mediators in germ-free and conventionally reared groups in both BALBc/ByJ and SCID C.B17 mice.Methods: Semiquantitative reverse transcription - polymerase chain reaction (RT-PCR) was utilized to determine the constitutive levels within each gingival tissue set (N = 5) for: E-selectin, P-selectin, interleukin-(IL)-8 homologue, tumor necrosis factor-alpha, IL-1beta, intercellular adhesion molecule-(ICAM)-1, ICAM-2, and vascular adhesion molecule- (VCAM)-1. In addition, IL-1 protein content was determined by enzyme-linked immunosorbent assay (ELISA).Results: Gingival samples revealed that only IL-1beta mRNA expression among all mediators examined was significantly reduced in conventionally reared mice (P