Effect of in vitro topophysis on the growth, development, and rooting of chrysanthemum explants (Chrysanthemum grandiflorum /Ramat./ Kitam)
Effect of in vitro topophysis on the growth, development, and rooting of chrysanthemum explants (Chrysanthemum grandiflorum /Ramat./ Kitam)
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体外拓扑结构对菊花外植体生长、发育和生根的影响（Chrysanthemum grandiflorum /Ramat./ Kitam）
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
A. Wenda
中科院分区：
文献类型：
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作者：
M. Zalewska;N. Miler;A. Wenda
总结使用离体，单节外植体从不同的拓扑位置在菊花（Chrysanthemum grandiflorum /Ramat./”（《易经》）。特别是外植体的位置对繁殖率的影响，对菊花微繁殖的成功具有重要意义。将五个菊花品种的试管苗均匀地分成三个相等的区：远心区、中心区和近心区。从每个区域分离两个单节外植体，并在不添加任何生长调节剂的MS培养基上培养。培养10周后，切除50%的从每个单节外植体上的腋芽发育的芽，并进行测量，以比较从来自不同地形物理区的外植体发育的芽。将剩余的芽在生根培养基上继代培养。4周后，记录每株植物的根数和根的总鲜重（FW）。品种分为两大类。'琥珀夫人'，'橙夫人'，和'夫人Vitroflora'外植体的拓扑结构依赖，而'青铜夫人'和'玫瑰夫人'外植体的拓扑结构独立。对于这三个地形依赖的品种，繁殖率，生长速度，芽长，节间长度，单叶重，和总植株FW值最高的那些芽来自中央和近端区。拓扑结构未能影响每枝的叶片数或两个连续叶片出现之间的天数。不同地理位置对单株根数和根重的影响不一致。菊花离体快繁过程中试管苗的不均匀生长可能是一种位置效应，这种现象在菊花中具有品种特异性。
Summary The consequences of using ex vitro, single-node explants from different topophysical positions in chrysanthemum (Chrysanthemum grandiflorum /Ramat./ Kitam) were determined. In particular, how explant topophysis affected the rate of propagation, which is important for the successful micropropagation of chrysanthemum. Uniform shoots of five cultivars of chrysanthemum, cultured in vitro, were each divided into three equal zones: distal, central, and proximal. Two single-node explants were isolated from each zone and cultured on MS medium without any added growth regulators. After 10 weeks of culture, 50% of the shoots that had developed from axillary buds on each single-node explant were excised and measurements were taken in order to compare those shoots that had developed from explants from the different topophysical zones. The remaining shoots were sub-cultured on rooting medium. After 4 weeks, the numbers of roots per plantlet, and the total fresh weight (FW) of roots were recorded. The cultivars fell into two groups. ‘Lady Amber’, ‘Lady Orange’, and ‘Lady Vitroflora’ explants were topophysis-dependent, while ‘Lady Bronze’ and ‘Lady Rosy’ explants were topophysis-independent. For the three topophysis-dependent cultivars, the propagation rate, growth rate, shoot length, internode length, single leaf weight, and total plantlet FW values were highest for those shoots derived from the central and proximal zones. Topophysis failed to affect the number of leaves per shoot or the number of days between the appearance of two successive leaves. The effects of topophysis on the number of roots per plantlet and on root FW were inconsistent. The unequal growth of chrysanthemum plantlets during in vitro micropropagation can be an effect of topophysis, and this phenomenon is cultivar-specific in chrysanthemum.