INDUCTION OF HEAT-SHOCK PROTEIN (HSP) BY MOXIBUSTION
INDUCTION OF HEAT-SHOCK PROTEIN (HSP) BY MOXIBUSTION
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DOI：
10.1142/s0192415x95000390
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发表时间：
1995-01-01
影响因子：
5.7
通讯作者：
KOBAYASHI, K
中科院分区：
文献类型：
--
作者：
KOBAYASHI, K
艾灸大鼠臀部肌肉穴位，肌内温度保持在40 ℃，持续15分钟。在深度麻醉下处死大鼠，并在刺激后立即、3小时和24小时切除肌肉组织。从刺激大鼠和对照大鼠的匀浆和离心组织中提取蛋白质。采用双向凝胶电泳技术，检测艾灸刺激后3 h处死大鼠的热休克蛋白（Heat-shock protein，hsp），其分子量分别为70，000（hsp 70）、85，000（hsp 85）和100，000（hsp 100）。分析蛋白质图谱，并获得hsps的比例。
Rats were treated by moxibustion at the point of hip muscle, and intramuscular temperature was kept at 40 degrees C for 15 minutes. The rats were sacrificed under deep anesthesia and the muscular tissues were excised immediately, three hours and 24 hours after stimulation. Proteins were extracted from the homogenized and centrifuged tissues of the stimulated rats and control rats. Two-dimensional gel electrophoresis of the proteins was carried out, Heat-shock protein (hsp) with molecular weight of 70,000 (hsp 70), 85,000 (hsp 85) and 100,000 (hsp 100) was detected in rats sacrificed three hours after the stimulation by moxibustion. Protein patterns were analyzed and the ratios of the hsps were obtained.