Six-stage cascade paramagnetic mode magnetophoretic separation system for human blood samples
Six-stage cascade paramagnetic mode magnetophoretic separation system for human blood samples
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DOI：
10.1007/s10544-010-9416-3
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发表时间：
2010-08-01
影响因子：
2.8
通讯作者：
Frazier, A. Bruno
中科院分区：
文献类型：
--
作者：
Jung, Youngdo;Choi, Yoonsu;Frazier, A. Bruno
基于血液中悬浮细胞的自然磁性，研制了一种六级串联顺磁模式磁泳分离系统。设计和制造的PMMS系统和微流控分离系统的特点是实验使用人体全血作为案例研究。PMMS系统可以使用由高磁场梯度产生的磁泳力连续分离血细胞类型，而无需磁性或荧光标记。实验结果表明，在PMMS系统中以28.8 μ L / hr的体积流速分离红细胞，导致5.0 μ L血液样品的分离时间为10.4分钟，分离效率为89.5 +/-0.20%。PMMS系统在50.4 μ L / hr和72.0 μ L /hr的较高体积流速下进行测试，测得的分离效率分别为86.2 +/- 1.60%和59.9 +/- 6.06%。
This paper is focused on the development of a six-stage cascade paramagnetic mode magnetophoretic separation (PMMS) system for separating suspended cells in blood based on their native magnetic properties. The design and fabrication of a PMMS system are presented and the microfluidic separation system is characterized experimentally using human whole blood as the case study. The PMMS system can separate blood cells types continuously using the magnetophoretic force produced from a high magnetic field gradient without magnetic or fluorescent tagging. Experimental results demonstrated that red blood cell separation in the PMMS system at a volumetric flow rate of 28.8 mu L / hr, resulting in a separation time of 10.4 min for a 5.0 mu L blood sample with a separation efficiency of 89.5 +/- 0.20%. The PMMS system was tested at higher volumetric flow rates of 50.4 mu L / hr and 72.0 mu L / hr. The measured separation efficiencies were 86.2 +/- 1.60% and 59.9 +/- 6.06% respectively.