Discriminative stimulus effects of ethanol and 3 alpha-hydroxy-5 alpha-pregnan-20-one in relation to menstrual cycle phase in cynomolgus monkeys (Macaca fascicularis)
Discriminative stimulus effects of ethanol and 3 alpha-hydroxy-5 alpha-pregnan-20-one in relation to menstrual cycle phase in cynomolgus monkeys (Macaca fascicularis)
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DOI：
10.1007/s002130050211
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发表时间：
1997-03-01
影响因子：
3.4
通讯作者：
Purdy, RH
中科院分区：
文献类型：
--
作者：
Grant, KA;Azarov, A;Purdy, RH
本研究的目的是表征的歧视性刺激效应的乙醇和神经甾体3 α-羟基-5 α-甾烷-20-酮（allopregnanolone）在非人灵长类动物作为月经周期阶段的函数。雌性食蟹猴（Macaca fascicularis）在两级程序中接受训练，以使用食物强化区分1.0 g/kg乙醇（IG，30 min预处理）和水。采用累积给药程序评估乙醇和内源性抗焦虑类固醇在月经周期卵泡期与黄体期的效力变化。血浆孕酮和别孕烯醇酮水平在测试的24小时内测定，以验证月经周期的阶段。在月经周期的黄体期，猴子对乙醇的辨别刺激作用和内源性神经活性类固醇别孕烯醇酮的乙醇样作用更敏感。这些研究结果表明，卵巢源性孕酮和allopregnanolone的内源性水平的变化改变敏感性的歧视性刺激乙醇的影响。
The present study was designed to characterize the discriminative stimulus effects of ethanol and the neurosteroid 3 alpha-hydroxy-5 alpha-pregnan-20-one (allopregnanolone) in nonhuman primates as a function of menstrual cycle phase. Female cynomolgus monkeys (Macaca fascicularis) were trained in a two-lever procedure to discriminate 1.0 g/kg ethanol (IG, 30 min pretreatment) from water using food reinforcement. A cumulative dosing procedure was used to assess changes in the potency of ethanol and an endogenous anxiolytic steroid in the follicular versus the luteal phase of the menstrual cycle. Plasma progesterone and allopregnanolone levels were determined within 24 h of testing to verify phase of menstrual cycle. The monkeys were more sensitive to the dis criminative stimulus effects of ethanol and the ethanol-like effects of the endogenous neuroactive steroid allopregnanolone during the luteal phase of the menstrual cycle. These findings suggest that changes in the endogenous levels of ovarian-derived progesterone and allopregnanolone alter sensitivity to the discriminative stimulus effects of ethanol.