A dimeric bispecific miniantibody combines two specificities with avidity
A dimeric bispecific miniantibody combines two specificities with avidity
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DOI：
10.1016/s0014-5793(98)00829-1
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发表时间：
1998-07-31
期刊：
影响因子：
3.5
通讯作者：
Plückthun, A
中科院分区：
文献类型：
--
作者：
Müller, KM;Arndt, KM;Plückthun, A
双特异性抗体扩展了自然界的能力，并可能应用于免疫治疗和生物技术。通过将单链抗体(ScFv)的基因融合到螺旋二聚结构域，然后再融合第二个不同特异性的scFv片段，我们能够在大肠杆菌中表达一个双特异性的、每个特异性有两个价态的功能蛋白，二聚体双特异性(DiBI)小抗体在一个只有120 kDa的非常小的分子中保持了抗体的天然亲和力，原理的共性在于一个scFv片段与EGF受体(命名为scFv 425)以三种组合与针对CD2(ACID2.M1)的scFv片段结合。磷酰胆碱(McPC603)或荧光素(FITC-ET)。用夹心表面等离子体共振生物传感器(Biacore)测量分析结合的MAS。(C)1998年欧洲生化学会联合会。
Bispecific antibodies extend the capabilities of nature and might be applied in immunotherapy and biotechnology. By fusing the gene of a single-chain Fv (scFv) fragment to a helical dimerization domain, followed by a second scFv fragment of different specificity, we were able to express a functional protein in E. coli, which is bispecific and has two valencies for each specificity, The dimeric bispecific (DiBi) miniantibody preserves the natural avidity of antibodies in a very small-sized molecule of only 120 kDa, The generality of the principle was shown with a scFv fragment binding the EGF-receptor (named scFv 425) in three combinations with scFv fragments tither directed against CD2 (ACID2.M1)? phosphorylcholine (McPC603) or fluorescein (FITC-Et). Binding mas analyzed by sandwich surface plasmon resonance biosensor (BIAcore) measurements. (C) 1998 Federation of European Biochemical Societies.