Detection of cytomegalovirus, human parvovirus B19, and herpes simplex virus‐1/2 in women with first‐trimester spontaneous abortions
Detection of cytomegalovirus, human parvovirus B19, and herpes simplex virus‐1/2 in women with first‐trimester spontaneous abortions
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DOI：
10.1002/jmv.24218
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发表时间：
2015-10
影响因子：
12.7
通讯作者：
Ya Zhou;Guohui Bian;Qiongxiu Zhou;Zhan Gao;P. Liao;Yu Liu;M. He
中科院分区：
文献类型：
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作者：
Ya Zhou;Guohui Bian;Qiongxiu Zhou;Zhan Gao;P. Liao;Yu Liu;M. He
病毒感染与妊娠早期自然流产之间的关系尚不清楚。本研究旨在通过分子和血清学技术研究妊娠早期自然流产女性中巨细胞病毒（CMV）、人细小病毒B19（B19 V）和单纯疱疹病毒1/2（HSV-1/2）感染的患病率。使用真实的实时定量聚合酶链反应（真实的实时qPCR）检查血浆样本中的CMV、B19 V和HSV-1/2 DNA，并使用血清学试验检查针对B19 V、CMV和HSV-1/2的特异性IgG抗体。流产组包括有两次或两次以上妊娠早期自然流产史的女性（n = 1，716）。年龄小于30岁的妇女发生自然流产的比例较高。无标本B19 V或CMV DNA阳性。1，716份标本中有7份HSV-1/2 DNA呈阳性。血清学检查B19 VIgG阳性率为47.24%，HSV IgG阳性率为39.66%，CMV IgG阳性率为79.31%，B19 VIgM阳性率为9.31%。CMV IgG的高阳性率表明，大多数妊娠早期自然流产的妇女对原发性CMV感染不敏感。大多数病例中缺乏病毒DNA表明B19 V，CMV和HSV-1/2感染通常与早期自然流产无关。J. Med. Virol. 87：1749-1753，2015.© 2015 Wiley Periodicals，Inc.
The relationship between viral infections and first‐trimester spontaneous abortions is not well‐understood. The study aim was to investigate the prevalence of cytomegalovirus (CMV), human parvovirus B19 (B19V), and herpes simplex virus‐1/2 (HSV‐1/2) infection by molecular and serological techniques in women experiencing spontaneous miscarriage in the first trimester of pregnancy. Plasma samples were examined for CMV, B19V, and HSV‐1/2 DNA using real‐time quantitative polymerase chain reaction (Real‐time qPCR), and for specific IgG antibodies against B19V, CMV, and HSV‐1/2 using serological assays. The abortion group consisted of women (n = 1,716) with a history of two or more first‐trimester spontaneous abortions. Women younger than 30 years possess higher portion to experience spontaneous abortion. No specimens were positive for B19V or CMV DNA. Seven out of the 1,716 specimens were positive for HSV‐1/2 DNA. By serology, 47.24% of patients were positive for B19V IgG, 39.66% for HSV IgG, 79.31% for CMV IgG, and 9.31% for B19V IgM. The high rate of positivity for CMV IgG suggests that the majority of women with first‐trimester spontaneous abortions are not susceptible to primary CMV infection. The lack of virus DNA in the majority of cases indicates that B19V, CMV, and HSV‐1/2 infection is not commonly associated with first‐trimester spontaneous abortion. J. Med. Virol. 87:1749–1753, 2015. © 2015 Wiley Periodicals, Inc.